Product Class: Kit

Q5® High-Fidelity PCR Kit

Product Introduction

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot Start and Master Mix formats available
Catalog # Size Concentration
E0555S 50.0 reactions
E0555L 200.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Protocol for a Routine PCR (E0555)
  2. PCR Optimization (E0555)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What are the advantages of using Q5® High-Fidelity DNA Polymerase?
  2. What are the differences between the numerous Q5® Polymerase products available?
  3. My results are not as expected. Where can I find troubleshooting help?
  4. What ends will my PCR products have?
  5. What does exonuclease activity mean for a DNA polymerase?
  6. Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
  7. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  8. How should I determine the appropriate annealing temperature for my reaction?
  9. What should the final primer concentration be in my PCR?
  10. When and how should I use the Q5® High GC Enhancer?
  11. What are the stability and storage requirements of the Q5® PCR Kit?
  12. There is a precipitate in the bottom of the buffer tube. Is this normal?

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