Q5® High-Fidelity DNA PolymeraseProduct information
Q5 High-Fidelity DNA Polymerase
|100 units ( 2000 units/ml )||
Q5 High-Fidelity DNA Polymerase
|500 units ( 2000 units/ml )||
Fidelity at its finest.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Featured VideosView Video Library
Important Tips for Q5 Polymerase
Why Choose Q5 High-fidelity Polymerase?
Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
Why is Tm Important in Primer Design?
Tips for Amplifying Large Amplicons
How to Amplify GC-rich DNA
5 Tips for Setting Up Your PCR
Overview of PCR
Q5® High-Fidelity DNA Polymerase
is a high-fidelity, thermostable DNA polymerase
with 3´→ 5´ exonuclease activity, fused to a
processivity-enhancing Sso7d domain to support
robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity
DNA Polymerase is ideal for cloning and can
be used for long or difficult amplicons. Q5
High-Fidelity DNA Polymerase is supplied with
an optimized buffer system that allows robust
amplification regardless of GC content. The 5X
Q5 Reaction Buffer contains 2 mM Mg++ at final
(1X) reaction concentrations and is recommended
for most routine applications. For GC-rich targets
(≥ 65% GC), amplification can be improved by
the addition of the 5X Q5 High GC Enhancer. Q5
High-Fidelity DNA Polymerase is unlike typical,
lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Product SourceAn E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Q5® Reaction Buffer Pack||-20||5 X|
|Q5® High GC Enhancer||-20||5 X|
- Product Categories:
- Q5® High-Fidelity DNA Polymerases Products
Advantages and Features
- High-fidelity PCR
- Long or Difficult Amplification
- High-throughput PCR
Properties & Usage
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.
Unit Assay Conditions25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
Protocols, Manuals & Usage
Usage & Guidelines
Tools & Resources
Faqs & Troubleshooting
- What are the advantages to using Q5® High-Fidelity DNA Polymerase?
- What is the fidelity of Q5® High-Fidelity DNA Polymerase?
- How should I determine an appropriate annealing temperature for my reaction?
- What should my primer concentration be when using Q5® High-Fidelity DNA Polymerase products?
- How should I set up a PCR experiment using Q5® High-Fidelity DNA Polymerase?
- My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
- Do I need to modify my annealing temperature when using the Q5® High GC Enhancer?
- When should I add the High GC Enhancer?
- Are the DNA fragments produced by Q5® High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields?
- There is a precipitate in the bottom of the buffer tube. Is this normal?
- What length of product can be made by Q5® High-Fidelity DNA Polymerase?
- I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
- Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
- Where can I find help troubleshooting my PCR?
- Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?
- I'd like to clone a fragment amplified with Q5® High-Fidelity DNA Polymerase. Do I have to blunt-end clone?
- Do other polymerases work in Q5® Reaction Buffer?
- I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
Citations & Technical Literature
- Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013) ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR) Molecular Pharmacology Fast Forward; 87247. PubMedID: 23907213
- Hicham Bouabe, Klaus Okkenhaug (2013) A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells Methods in Molecular Biology; 1064, 337-354. PubMedID: 23996269
- Wilber Quispe-Tintaya, Ryan R White, Vasily N Popov, Jan Vijg, Alexander Y Maslov (2013) Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing Biotechniques; 55(3), 133-6. PubMedID: 24003945 , DOI: 10.2144/000114077
- Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc (2013) Hedgehog Signaling Regulates MyoD Expression and Activity J Biol Chem; 288(6), 4389–4404. PubMedID: 23266826
- Christine Henke, Pamela L Strissel, Maria-Theresa Schubert, Megan Mitchell, Claus C Stolt, Florian Faschingbauer, Matthias W Beckmann, Reiner Strick (2015) Selective expression of sense and antisense transcripts of the sushi-ichi-related retrotransposon - derived family during mouse placentogenesis. Retrovirology; 12, 9. PubMedID: 25888968 , DOI: 10.1186/s12977-015-0138-8
- Amin Zargar, David N Quan, Milad Emamian, Chen Yu Tsao, Hsuan-Chen Wu, Chelsea R Virgile, William E Bentley (2015) Rational design of 'controller cells' to manipulate protein and phenotype expression. Metab Eng; , PubMedID: 25908186 , DOI: 10.1016/j.ymben.2015.04.001
- Silva-Herzog E, McDonald EM, Crooks AL, Detweiler CS. (2015) Physiologic Stresses Reveal a Salmonella Persister State and TA Family Toxins Modulate Tolerance to These Stresses PLoS One; 12, PubMedID: 26633172 , DOI: 10.1371
- Yang YJ, Han YY, Chen K, Zhang Y, Liu X, Li S, Wang KQ, Ge JB, Liu W, Zuo J. (2015) TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes Cell Death Dis.; PubMedID: 26673669 , DOI: 10.1038
- Yuan Xue, Jossef Osborn, Anand Panchal, Jay L Mellies (2015) The RpoE Stress Response Pathway Mediates Reduction of the Virulence of Enteropathogenic Escherichia coli by Zinc. Appl Environ Microbiol; 81, 3766-74. PubMedID: 25819956 , DOI: 10.1128/AEM.00507-15
- Harish Nag Kankipati, Marta Rubio-Texeira, Dries Castermans, George Diallinas, Johan M Thevelein (2015) Sul1 and Sul2 Sulfate Transceptors Signal to Protein Kinase A upon Exit of Sulfur Starvation. J Biol Chem; 290, 10430-46. PubMedID: 25724649 , DOI: 10.1074/jbc.M114.629022
- Binyamin D Berkovits, Christine Mayr (2015) Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature; , PubMedID: 25896326 , DOI: 10.1038/nature14321
- Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He, Zhongwei Li, Chris Benner, Isao Tamura, Marie N Krause, Joseph R Nery, Tingting Du, Zhuzhu Zhang, Tomoaki Hishida, Yuta Takahashi, Emi Aizawa, Na Young Kim, Jeronimo Lajara, Pedro Guillen, Josep M Campistol, Concepcion Rodriguez Esteban, Pablo J Ross, Alan Saghatelian, Bing Ren, Joseph R Ecker, Juan Carlos Izpisua Belmonte (2015) An alternative pluripotent state confers interspecies chimaeric competency. Nature; , PubMedID: 25945737 , DOI: 10.1038/nature14413
- Longhai Dai, Can Liu, Yueming Zhu, Jiangsheng Zhang, Yan Men, Zeng Yan, Yuanxia Sun (2015) Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii. Plant Cell Physiol; , PubMedID: 25759326 , DOI: 10.1093/pcp/pcv043
- Yonghe Zhang, Huiming Huang, Shanshan Xu, Bo Wang, Jianhua Ju, Huarong Tan, Wenli Li (2015) Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact; 14, 64. PubMedID: 25927229 , DOI: 10.1186/s12934-015-0244-2
- Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal, Jiri Hulcr (2014) The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing. ISME J; , PubMedID: 25083930 , DOI: 10.1038/ismej.2014.115
- Vidhyadhar Nandana, Sushant Singh, Abhay Narayan Singh, Vikash Kumar Dubey (2014) Procerain B, a cysteine protease from Calotropis procera, requires N-terminus pro-region for activity: cDNA cloning and expression with pro-sequence. Protein Expr Purif; 103C, 16-22. PubMedID: 25173974 , DOI: 10.1016/j.pep.2014.08.003
- Xin Duan, Arjun Krishnaswamy, Irina De la Huerta, Joshua R Sanes (2014) Type II Cadherins Guide Assembly of a Direction-Selective Retinal Circuit. Cell; 158, 793-807. PubMedID: 25126785 , DOI: 10.1016/j.cell.2014.06.047
- Bert De Rybel, Milad Adibi, Alice S. Breda, Jos R. Wendrich, Margot E. Smit, Ondej Novk, Nobutoshi Yamaguchi, Saiko Yoshida, Gert Van Isterdael, Joakim Palovaara, Bart Nijsse, Mark V. Boekschoten, Guido Hooiveld, Tom Beeckman, Doris Wagner, Karin Ljung, Christian Fleck, Dolf Weijers (2014) Integration of growth and patterning during vascular tissue formation in Arabidopsis Science; 345, 1255215. PubMedID: 25104393 , DOI: 10.1126/science.1255215
- Wang XJ, Zhang XJ, Hu W, Zhang TY, Wang SQ (2014) A simple and efficient strategy for the de novo construction of greater-than-genome-length hepatitis B virus replicons J Virol Methods; 207, 158-62. PubMedID: 25025817 , DOI: 10.1016/j.jviromet.2014.07.009
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Q5® High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
Q5® High GC Enhancer
Legal and DisclaimersThis product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product is covered by one or more Patents.
This product is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (not real-time) PCR in the research field only, but not real time PCR or digital PCR; (b) real-time PCR for use as a library preparation quantitation tool in Next Generation Sequencing workflows; (c) any in-vitro diagnostics applications, except for applications using real-time PCR or digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification, and the production of synthetic DNA.
Q5® is a registered trademark of New England Biolabs, Inc.
The supporting documents available for this product can be downloaded below.