Product Class: Other

Deoxynucleotide (dNTP) Solution Mix

Product Introduction

Enhance your PCR Reaction with our ultrapure dNTPs

  • Compatible with all NEB DNA polymerases in a variety of applications
  • ≥ 99% pure as determined by HPLC analysis
  • Exceptional stability
Catalog # Size Concentration
N0447S 8 μmol of each 10 mM each nt
N0447L 40 μmol of each 10 mM each nt

Product Information

Description

Deoxynucleotide (dNTP) Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP.

Each vial contains 8 μmol in a volume of 0.8 mls. A small pack contains 4 vials of 0.2 mls and a large pack contains 5 vials of 0.8 mls.

Supplied in: Ultrapure water as a sodium salt at pH 7.5.
Product Categories:
Nucleotide Solution Products,
Reverse Transcriptases & RT-PCR Products
Applications:
Whole Genome Amplification,
Polymerases for DNA Manipulation,
PCR
,
DNA Sequencing
,
Isothermal Amplification
,
Hot Start PCR
,
RT-PCR & cDNA Synthesis
,
Long Range PCR
,
Fast PCR
,
Methylated DNA Analysis
,
Multiplex PCR
,
PCR
,
Specialty PCR
,
Routine PCR

Properties and Usage

Storage Temperature

-20°C

Notes

  1. Can be diluted using strerile distilled water, preferably milli-q® water, or sterile TE (10mM Tris-HCl, 1mM EDTA pH 7.5).

Faqs & Tech Tips

FAQs

  1. What is supplied with the Deoxynucleotide Solution Mix?
  2. What is in the Deoxynucleotide Solution Mix?
  3. How stable are the nucleotides?
  4. What is the concentration of Deoxynucleotide Solution Mix?
  5. What polymerases can the Deoxynucleotide Solution Mix be used with?
  6. How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
  7. If a final concentration of 200 μM each dNTP is called for, how much of the mix should be added to a 100 μl reaction?

Protocols & Manuals

Protocols

  1. Loop-mediated Isothermal Amplification (LAMP)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.