Product Class: Other

Q5® Reaction Buffer Pack

Product Introduction

Superior performance with minimal optimization

  • Creates optimal conditions for amplification with Q5® and Q5 Hot Start High-Fidelity DNA Polymerases
  • Can be coupled with Q5 High GC Enhancer to amplify high GC (>= 65%) amplicons
  • 5X concentration
Bioz Badge Exists : True

Product Information

Description

  

New England Biolabs supplies a 5X reaction buffer and supplemental reagent for use with Q5® High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.

Properties & Usage

Storage Temperature

-20°C

Product Notes

  1. Reaction Conditions:
    At a 1X concentration the reaction buffer contains 2 mM Mg++ and assures optimal activity for Q5 High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.

References

  1. Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404. PubMedID: PMC3567689,
  2. Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE 
    PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION 
    OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247,
  3. Hicham Bouabe, Klaus Okkenhaug (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology. 1064, 337-354.

Protocols, Manuals & Usage

Usage & Guidelines

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Do I need to modify my annealing temperature when using the Q5® High GC Enhancer?
  2. When should I add the High GC Enhancer?
  3. There is a precipitate in the bottom of the buffer tube. Is this normal?
  4. Do other polymerases work in Q5® Reaction Buffer?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

This product is covered by one or more Patents.

Trademarks



Q5® is a registered trademark of New England Biolabs, Inc.