Product Class: Other

Q5® Reaction Buffer Pack

Product Introduction

Superior performance with minimal optimization

  • Creates optimal conditions for amplification with Q5® and Q5 Hot Start High-Fidelity DNA Polymerases
  • Can be coupled with Q5 High GC Enhancer to amplify high GC (>= 65%) amplicons
  • 5X concentration
Catalog # Size Concentration
B9027S 6.0 ml 5 X

Product Information



New England Biolabs supplies a 5X reaction buffer and supplemental reagent for use with Q5® High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.

This product is related to the following categories:
Reaction Buffers Products,
Q5® High-Fidelity DNA Polymerases Products,
Buffers Products,
This product can be used in the following applications:
Long Range PCR,
Fast PCR,
High-Fidelity PCR,
Multiplex PCR,
Specialty PCR,
Routine PCR,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • B9027S     -20    

Properties & Usage

Product Notes

  1. Reaction Conditions:
    At a 1X concentration the reaction buffer contains 2 mM Mg++ and assures optimal activity for Q5 High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.


  1. Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404.
  2. Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE 
    OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247,
  3. Hicham Bouabe, Klaus Okkenhaug (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology. 1064, 337-354.

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting


  1. Do other polymerases work in Q5® Reaction Buffer?
  2. There is a precipitate in the bottom of the Master Mix tube? Is this normal?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.


The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.


This product is covered by one or more Patents.


Q5® is a registered trademark of New England Biolabs, Inc.