Q5® Hot Start High-Fidelity DNA PolymeraseProduct information
Q5 Hot Start High-Fidelity DNA Polymerase
|100 units ( 2,000 units/ml )||-||Unavailable in your region|
Q5 Hot Start High-Fidelity DNA Polymerase
|500 units ( 2,000 units/ml )||-||Unavailable in your region|
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Featured VideosView Video Library
Important Tips for Q5 Polymerase
Why Choose Q5 High-fidelity Polymerase?
Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
Why is Tm Important in Primer Design?
Tips for Amplifying Large Amplicons
How to Amplify GC-rich DNA
5 Tips for Setting Up Your PCR
Overview of PCR
Q5® Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Product SourceAn E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
Advantages and Features
- High-specificity PCR
- High-fidelity PCR
- Long or Difficult Amplification
- High-throughput PCR
Properties & Usage
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.
Unit Assay Conditions25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
Protocols, Manuals & Usage
Usage & Guidelines
Tools & Resources
FAQs & Troubleshooting
- What are the advantages to using Q5® Hot Start High-Fidelity DNA Polymerase?
- What is the fidelity of Q5® High-Fidelity DNA Polymerase?
- How should I determine an appropriate annealing temperature for my reaction?
- What should my primer concentration be when using Q5® High-Fidelity DNA Polymerase products?
- How should I set up a PCR reaction using Q5® Hot Start High-Fidelity DNA Polymerase?
- My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
- Do I need to modify my annealing temperature when using the Q5® High GC Enhancer?
- When should I add the High GC Enhancer?
- How do I activate Q5® Hot Start High-Fidelity DNA Polymerase?
- Are the DNA fragments produced by Q5® High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields?
- There is a precipitate in the bottom of the buffer tube. Is this normal?
- What length of product can be made by Q5® High-Fidelity DNA Polymerase?
- I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
- Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
- Where can I find help troubleshooting my PCR?
- Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?
- I'd like to clone a fragment amplified with Q5® High-Fidelity DNA Polymerase. Do I have to blunt-end clone?
- Do other polymerases work in Q5® Reaction Buffer?
- What is the difference between Q5® and Q5U™ Hot Start High-Fidelity DNA Polymerase?
Citations & Technical Literature
- Toru Takahashi, Ken Maeda, Tadaki Suzuki, Aki Ishido, Toru Shigeoka, Takayuki Tominaga, Toshiaki Kamei, Masahiro Honda, Daisuke Ninomiya, Takenori Sakai, Takanori Senba, Shozo Kaneyuki, Shota Sakaguchi, Akira Satoh, Takanori Hosokawa, Yojiro Kawabe, Shintaro Kurihara, Koichi Izumikawa, Shigeru Kohno, Taichi Azuma, Koichiro Suemori, Masaki Yasukawa, Tetsuya Mizutani, Tsutomu Omatsu, Yukie Katayama, Masaharu Miyahara, Masahito Ijuin, Kazuko Doi, Masaru Okuda, Kazunori Umeki, Tomoya Saito, Kazuko Fukushima, Kensuke Nakajima, Tomoki Yoshikawa, Hideki Tani, Shuetsu Fukushi, Aiko Fukuma, Momoko Ogata, Masayuki Shimojima, Noriko Nakajima, Noriyo Nagata, Harutaka Katano, Hitomi Fukumoto, Yuko Sato, Hideki Hasegawa, Takuya Yamagishi, Kazunori Oishi, Ichiro Kurane, Shigeru Morikawa, Masayuki Saijo (2013) The First Identification and Retrospective Study of Severe Fever With Thrombocytopenia Syndrome in Japan The Journal of Infectious Diseases; 209(6), 816-827. PubMedID: 24231186
- Stoczynska-Fidelus E, Och W, Rieske P, Bienkowski M, Banaszczyk M, Winiecka-Klimek M, Zieba J, Janik K, Rosiak K, Treda C, Stawski R, Radomiak-Zaluska A, Piaskowski S (2014) Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H Anticancer Res; 34(6), 2859-67. PubMedID: 24922649
- Currin A, Swainston N, Day PJ, Kell DB (2014) SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution Protein Eng Des Sel; 27(9), 273-80. PubMedID: 25108914 , DOI: 10.1093/protein/gzu029
- Juliane Krebes, Richard D. Morgan, Boyke Bunk, Cathrin Spröer, Khai Luong, Raphael Parusel, Brian P. Anton, Christoph König, Christine Josenhans, Jörg Overmann, Richard J. Roberts, Jonas Korlach, Sebastian Suerbaum (2013) . The complex methylome of the human gastric pathogen Helicobacter pylori Nucleic Acids Research.; 42(4), 2415-2432. PubMedID: 24302578 , DOI: 10.1093/nar/gkt1201
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
Q5® High GC Enhancer
Legal and DisclaimersThis product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product is covered by one or more Patents.
This product is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (not real-time) PCR in the research field only, but not real time PCR or digital PCR; (b) real-time PCR for use as a library preparation quantitation tool in Next Generation Sequencing workflows; (c) any in-vitro diagnostics applications, except for applications using real-time PCR or digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification, and the production of synthetic DNA.
TrademarksNEW ENGLAND BIOLABS® and Q5® are registered trademarks of New England Biolabs, Inc.
LABCHIP® is a registered trademark of Caliper Life Sciences, Inc., part of PerkinElmer, Inc.
SYBR® is a registered trademark of Molecular Probes, Inc., now owned by Life Technologies, Inc.
The supporting documents available for this product can be downloaded below.