Gel Loading Dye, Purple (6X) is a
pre-mixed loading buffer which contains a combination
of two dyes, Dye 1 (pink/red) and Dye 2
(blue). The red dye serves as the tracking dye for
both agarose and non-denaturing polyacrylamide
gel electrophoresis. The two dyes separate upon
gel electrophoresis; the red band is the major
indicator and migrates similarly to Bromophenol
Blue on agarose gels. Specifically chosen, this
dye does not leave a shadow under UV light. This
solution contains SDS, which often results in
sharper bands, as some restriction enzymes are
known to remain bound to DNA following cleavage.
EDTA is also included to chelate magnesium
(up to 10 mM) in enzymatic reactions, thereby
stopping the reaction. The dye also contains Ficoll,
which creates brighter and tighter bands when
compared to glycerol loading dyes. This product is packaged as 4x1 ml vials.
Attention SYBR® Safe and GelRed™ dye users: Due to an increased concentration of SDS* in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
SYBR® is a registered trademark of Life Technologies Corp. GelRed™ is a trademark of Biotium.