Product Class: Other

Gel Loading Dye, Blue (6X)

Product Introduction

Gel Loading Dye, Blue (6X) is a Bromophenol Blue-based loading dye offering convenient gel loading and sharp bands.

  • For use with agarose and non-denaturing polyacrilamide gels
  • Contains SDS for improved band sharpness
  • Contains EDTA to halt enzymatic reactions

You may also be interested in our Gel Loading Dye, Purple (6X) with and without SDS, for sharp bands and no UV shadow.

Catalog # Size Concentration
B7021S 4 ml 0 %

Product Information


Gel Loading Dye, Blue (6X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol Blue is the standard tracking dye for electrophoresis. It migrates at approximately 300 bp on a standard 1% TBE agarose gel. This product is packaged as 4x1 ml vials.
Product Categories:
Gel Loading Buffers,
Buffer Products

Properties and Usage

Storage Temperature


1X Master Mix Composition

2.5% Ficoll®-400pH 8 @ 25°C11 mM EDTApH 8 @ 25°C3.3 mM Tris-HClpH 8 @ 25°C0.017% SDSpH 8 @ 25°C0.015% bromophenol bluepH 8 @ 25°C

1X Master Mix Composition

2.5% Ficoll®-400 (pH 0 @ 0°C)pH 0 @0°C11mM EDTA (pH 0 @ 0°C)pH 0 @0°C3.3mM Tris-HCl (pH 0 @ 0°C)pH 0 @0°C0.017% SDS (pH 0 @ 0°C)pH 0 @0°C0.015% bromophenol blue (pH 0 @ 0°C)pH 0 @0°C


  1. Use 5 µl of Gel Loading Dye, Blue (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.

Faqs & Tech Tips


  1. Are Gel Loading Dye, Blue (6x) and Gel Loading Dye Orange (6x) compatible with other systems such as SYBR (add registered mark symbol) and GelRed during gel electrophoresis?
  2. How are the 6x loading dyes packaged?

Protocols & Manuals


The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Usage Guildelines & Tips

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.


The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.