Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands.
No UV shadow
Contains Ficoll® for brighter, tighter bands
Contains EDTA to stop enzymatic reactions
Compatible with agarose and non-denaturing polyacrylamide gels
Our Purple Gel Loading Dye sharpens bands and eliminates the UV shadow seen with other dyes. Available with SDS (NEB #B7024S) or without SDS
Product Information
Gel Loading Dye, Purple (6X), no SDS is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band
is the major indicator and migrates similarly to Bromophenol Blue on agarose gels. Specifically chosen, this dye does not leave a shadow under UV light. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll®, which creates brighter and tighter bands when compared to glycerol loading dyes. This product is packaged as 4x1 ml vials.
Figure 1: Comparison of dye fronts
Nucleic acid samples were run on an agarose gel, with either Purple or Blue loading dyes. Both dyes ran at the same speed. Figure 2: UV shadow comparison
The new Gel Loading Dye, Purple (6X), no SDS, (Lane 1) included in the Quick-Load Purple 1kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).
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Specifications & Change Notifications
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