Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands.
No UV shadow
Contains Ficoll® for brighter, tighter bands
Contains EDTA to stop enzymatic reactions
Compatible with agarose and non-denaturing polyacrylamide gels
Our Purple Gel Loading Dye sharpens bands and eliminates the UV shadow seen with other dyes. Available with SDS (NEB #B7024S) or without SDS
Product Information
Gel Loading Dye, Purple (6X), no
SDS is a pre-mixed loading buffer which contains
a combination of two dyes, Dye 1 (pink/red) and
Dye 2 (blue). The red dye serves as the tracking
dye for both agarose and non-denaturing
polyacrylamide gel electrophoresis. The two dyes
separate upon gel electrophoresis; the red band
is the major indicator and migrates similarly
to Bromophenol Blue on agarose gels. Specifically
chosen, this dye does not leave a shadow under UV light. EDTA is also included to chelate
magnesium (up to 10 mM) in enzymatic reactions,
thereby stopping the reaction. The dye also
contains Ficoll®, which creates brighter and tighter
bands when compared to glycerol loading dyes. This product is packaged as 4x1 ml vials. Comparison of dye fronts
Nucleic acid samples were run on an agarose gel, with either Purple or Blue loading dyes. Both dyes ran at the same speed.UV shadow comparison
The new Gel Loading Dye, Purple (6X), no SDS, (Lane 1) included in the Quick-Load Purple 1kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).
This product is related to the following categories:
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Specifications & Change Notifications
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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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