Product Class: Other

1 kb DNA Ladder

This product is available in Quick-Load Purple format - enjoy the convenience of a ready-to-load format.

Product Introduction

  • Size range: 500 bp to 10 kb     
  • Value pricing
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

 

Catalog # Size Concentration
N3232S 200.0 gel lanes (0.2 ml) 500 µg/ml
N3232L 1000.0 gel lanes (1.0 ml) 500 µg/ml

Protocols, Manuals & Usage

Protocols

  1. End-labeling Protocol
  2. Suggested Loading Protocol for DNA Ladders & Markers

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Why are there extra bands visible on polyacrylamide gels?
  2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  3. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  4. How can I quantify the amount of DNA in each band of a marker?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  6. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  7. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

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