Product Class: Other

Quick-Load® 1 kb Plus DNA Ladder

This product was previously named the Quick-Load® 2-Log DNA Ladder. There have been no changes to product formulation or specifications.This product is available in Quick-Load Purple format - enjoy sharper bands, no UV shadow, and a lower price. 

Product Introduction

  • Size range:  100 bp to 10 kb
  • Convenient, ready-to-load format
  • Formulated with bromophenol blue dye
  • Easy to identify reference bands
  • Suitable for 250 gel lanes
Catalog # Size Concentration
N0469L 3.75 ml 100 μg/ml
N0469S 1.25 ml 100 μg/ml

Product Information

Description

Quick-Load® 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

NEB also offers a Quick-Load version of this ladder with purple dye.

  • Recommended gel percentage range: 1-1.4%
  • Optimum separation on 1.2% 

N0469_thumb
1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.

Mass values are for 1 µg/lane.

Suggested Load: 5 to 10 µl/lane.
Product Categories:
DNA Markers & Ladders Products
Applications:
DNA Analysis

Properties and Usage

Bases

Fragment Mass bp
1 40 10,002
2 40 8,001
3 48 6,001
4 40 5,001
5 32 4,001
6 120 3,001
7 40 2,017
8 57 1,517
9 45 1,200
10 122 1,000
11 34 900
12 31 800
13 27 700
14 23 600
15 124 500 / 517
16 49 400
17 37 300
18 32 200
19 61 100

Effective Size Range

100.0bp to 10,002.0bp

Storage Temperature

4°C

Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

Notes

  1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. In general, it is recommended to load 1µl per mm of gel lane.
  3. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  4. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
  5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.

Faqs & Tech Tips

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Can I use GelRed® with the DNA Ladders from NEB?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

Mix well before use, especially after thawing.

Protocols & Manuals

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Selection Charts

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.