Product Class: Nucleic Acid Marker

Quick-Load® 2-Log DNA Ladder (0.1-10.0 kb)

This product is available in Quick-Load Purple format - enjoy sharper bands, no UV shadow, and a lower price.
Catalog #SizeConcentrationGel Lanes
N0469S1.25 ml100 μg/ml250


Quick-Load® 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

NEB also offers a Quick-Load version of this ladder with purple dye.


Quick-Load 2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane.

Properties and Usage


15124500 / 517

Effective Size Range

100bp to 10,002bp

Storage Temperature


Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

Supporting Documents

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.


  1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load 2-Log DNA Ladder per gel lane.
  3. In general, it is recommended to load 1µl per mm of gel lane.
  4. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  5. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
  6. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.


  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.
  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® with the DNA Ladders from NEB?

Selection Charts

Mix well before use, especially after thawing.