Product Class: Other

100 bp DNA Ladder

 This product is available in Quick-Load Purple format - enjoy the convenience of a ready-to-load format.

Product Introduction

  • Size range: 100  bp - 1,517 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy-to-identify reference bands
  • Value pricing
  • Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
Catalog # Size Concentration
N3231S 100.0 gel lanes (0.1 ml) 500 µg/ml
N3231L 500.0 gel lanes (0.5 ml) 500 µg/ml

Protocols, Manuals & Usage

Protocols

  1. End-labeling Protocol
  2. Suggested Loading Protocol for DNA Ladders & Markers

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Why are there extra bands visible on polyacrylamide gels?
  2. Why is the separation of the lower bands incomplete?
  3. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  4. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  5. How can I quantify the amount of DNA in each band of a marker?
  6. Can I use Midori Green with the DNA Ladders from NEB?
  7. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  8. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
  9. What are the differences in the four versions of the 1 kb Plus (formerly called the 2-Log DNA Ladder), 100 bp and 1 kb DNA Ladders that NEB sells?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

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