Product Class: Other

pBR322 DNA-MspI Digest

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.

Product Introduction

  • Size range: 9 bp - 622 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Small size suitable for 50 gel lanes

 

Catalog # Size Concentration
N3032S 50.0 gel lanes (0.05 ml) 1000 µg/ml
N3032L 250.0 gel lanes (0.25 ml) 1000 µg/ml

Protocols, Manuals & Usage

Protocols

  1. Suggested Loading Protocol for DNA Ladders & Markers

     

FAQs & Troubleshooting

FAQs

  1. Why is the separation of the lower bands incomplete?
  2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  3. How can I quantify the amount of DNA in each band of a marker?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  6. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

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