Product Class: Nucleic Acid Marker

λ DNA-BstEII Digest

Now comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (B7025).
Catalog #SizeConcentrationGel Lanes
N3014S0.3 ml500 μg/ml150
N3014L1.5 ml500 μg/ml750

Description

The BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



N3014_thumb

Lambda DNA-BstE II Digest visualized by ethidium bromide staining. 1.0% agarose gel.
The full list of DNA fragments can be found in the chart below.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Gel Loading Dye, Purple (6X), no SDS256X

Properties and Usage

Bases

FragmentMassbp
11748,454
21497,242
31316,369
41175,686
5994,822
6894,324
7763,675
8482,323
9401,929
10281,371
11261,264
1214702
135224
142117

Effective Size Range

117bp to 8,454bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Supporting Documents

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
  2. The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
  3. 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Daniels, D.L. et al (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
  2. Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.
  1. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
  4. Can I use GelRed with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  6. Can I use SYBR® with the DNA Ladders from NEB?
  1. Suggested protocol for loading a sample

Heat at 60C for 3 minutes to separate the cohesive ends of fragments 1 and 4.

To make it ready-to-load, dilute in TE buffer instead of water.