Product Class: Other

λ DNA-BstEII Digest

 

Product Introduction

  • Size range: 117 bp to 8,454 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)

 

Catalog # Size Concentration
N3014L 1.5 ml 500 μg/ml
N3014S 0.3 ml 500 μg/ml

Product Information

Description

The BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

  • Recommended gel percentage range: 0.6-1.2%
  • Optimum separation on 0.8% 

N3014_thumb
Lambda DNA-BstE II Digest visualized by ethidium bromide staining. 1.0% agarose gel. The full list of DNA fragments can be found in the chart below.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
Gel Loading Dye, Purple (6X), no SDS 25 6 X
Product Categories:
DNA Markers & Ladders Products
Applications:
DNA Analysis

Properties and Usage

Bases

Fragment Mass bp
1 174 8,454
2 149 7,242
3 131 6,369
4 117 5,686
5 99 4,822
6 89 4,324
7 76 3,675
8 48 2,323
9 40 1,929
10 28 1,371
11 26 1,264
12 14 702
13 5 224
14 2 117

Effective Size Range

117.0bp to 8,454.0bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Notes

  1. The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
  2. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
  3. 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Daniels, D.L. et al (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
  2. Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.

Faqs & Tech Tips

FAQs

  1. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
  4. Can I use GelRed® with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  6. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

Heat at 60C for 3 minutes to separate the cohesive ends of fragments 1 and 4.

To make it ready-to-load, dilute in TE buffer instead of water.

Protocols & Manuals

Protocols

  1. Suggested Loading Protocol for N3014 & N3012

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.