λ DNA-BstEII DigestProduct information
DescriptionThe BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Gel Loading Dye, Purple (6X), no SDS||25||6X|
Properties and Usage
Effective Size Range117bp to 8,454bp
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
- For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
- The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
- 1X Gel Loading Dye, Purple, no SDS:
3.3mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2
- Daniels, D.L. et al (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
- Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- How can I quantify the amount of DNA in each band of a marker?
- Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
- Can I use GelRed with the DNA Ladders from NEB?
- Can I use Midori Green with the DNA Ladders from NEB?
- Can I use SYBR® with the DNA Ladders from NEB?
Heat at 60C for 3 minutes to separate the cohesive ends of fragments 1 and 4.
To make it ready-to-load, dilute in TE buffer instead of water.