LongAmp® Hot Start Taq DNA PolymeraseProduct information
LongAmp® Hot Start Taq DNA Polymerase offers exceptional performance with long amplicons.
- Aptamer-based inhibitor enables room temperature reaction setup
- Optimized enzyme blend for amplification up to 30 kb
- 2X higher fidelity than Taq
- Master mix version available
Tips for Amplifying Large Amplicons
Why is Tm Important in Primer Design?
5 Tips for Setting Up Your PCR
Overview of PCR
LongAmp Hot Start Taq DNA Polymerase is a unique blend of aptamer-based Hot Start Taq and Deep VentR™ DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The 3´→5´ exonuclease activity of Deep VentR DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase (1). LongAmp Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|LongAmp® Taq Reaction Buffer Pack||-20||5 X|
- Product Categories:
- LongAmp® DNA Polymerases
Advantages and Features
Properties and Usage
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
1X LongAmp™ Taq Reaction Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C
5' - 3' ExonucleaseYes
3' - 5' ExonucleaseYes
Unit Assay Conditions1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
Error Rate<= 0x100bases
- 5'→3' flap endonuclease destroys displaced strand.
- Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
- Saiki R.K. et al. (1985). Science. 230, 1350-1354.
- Powell, L.M. et al. (1987). Cell. 50, 831-840.
- Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
- Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
Faqs & Tech Tips
- What is the recommended enzyme amount when using LongAmp™ Hot Start Taq DNA Polymerase?
- What is the fidelity of the LongAmp™ Hot Start Taq DNA Polymerase compared to Taq DNA Polymerase?
- Can the extension step be carried out at 72°C when using LongAmp™ Hot Start Taq DNA Polymerase?
- What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Hot Start Taq DNA Polymerase?
- What is the extension rate when using LongAmp™ Hot Start Taq DNA Polymerase?
- Why is the product a smear when visualized on an agarose gel?
- Can LongAmp™ Hot Start Taq DNA Polymerase be used to amplify GC-rich amplicons?
Protocols & Manuals
Other Tools & Resources
Usage Guildelines & Tips
Troubleshooting GuidesPCR Troubleshooting Guide Taq PCR Kit Troubleshooting Guide
Quality & Safety
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
LongAmp® Hot Start Taq DNA Polymerase
LongAmp® Taq Reaction Buffer Pack
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
LicensesNotice to Purchaser:
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based LongAmp Hot Start Taq DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based LongAmp Hot Start Taq DNA Polymerase requires a license from New England Biolabs, Inc. Please contact email@example.com for more information.
TrademarksLONGAMP® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
DEEP VENT™ is a trademark of New England Biolabs, Inc.
IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is registered trademark of Uniqema Americas LLC.