Product Class: Kit

LongAmp® Taq PCR Kit

Product Introduction

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb from low complexity templates and difficult templates, such has human genomic DNA
  • 2X higher fidelity than Taq
  • Kit contains 5X Reaction Buffer for optimal performance
Catalog # Size Concentration
E5200S 100.0 reactions (50.0 µl)

Protocols, Manuals & Usage

Protocols

  1. Protocol for LongAmp™ Taq PCR Kit

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the recommended enzyme amount when using LongAmp™ Taq DNA Polymerase?
  2. Can the extension step be carried out at 72°C when using LongAmp™ Taq DNA Polymerase?
  3. What is the extension rate when using LongAmp™ Taq DNA Polymerase?
  4. Why is the product a smear when visualized on an agarose gel?
  5. Can the LongAmp™ kit be used to amplify GC-rich amplicons?
  6. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Taq DNA Polymerase?
  7. What is the fidelity of the LongAmp™ Taq DNA Polymerase compared to Taq DNA Polymerase?
  8. Can NEBuilder® HiFi DNA Assembly master mix remove both 3′ and 5′ end mismatches? 

Troubleshooting