OneTaq® DNA Polymerase
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M0480S |
OneTaq DNA Polymerase |
200 units ( 5000 units/ml ) | - | Unavailable in your region | |
M0480L |
OneTaq DNA Polymerase |
1000 units ( 5000 units/ml ) | - | Unavailable in your region | |
M0480X |
OneTaq DNA Polymerase |
5000 units ( 5000 units/ml ) | - | Unavailable in your region |
OneTaq® DNA Polymerase
Product Introduction
The ONE polymerase for your endpoint PCR needs. OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- 2X higher fidelity than Taq
- Ideal for routine, AT- or GC-rich templates
- Hot start and master mix versions available
Catalog # | Size | Concentration |
---|---|---|
M0480S | 200.0 units | 5000 units/ml |
M0480L | 1000.0 units | 5000 units/ml |
M0480X | 5000.0 units | 5000 units/ml |
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Product Information
Description
The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1) . The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.- This product is related to the following categories:
- OneTaq® DNA Polymerases Products,
- Taq DNA Polymerase Products,
- This product can be used in the following applications:
- Multiplex PCR,
- Specialty PCR,
- Routine PCR, PCR
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.Reaction Conditions
1X OneTaq® Standard Reaction Buffer Pack
1X OneTaq® Standard Reaction Buffer Pack
20 mM Tris-HCl
22 mM NH4Cl
22 mM KCl
1.8 mM MgCl2
0.06% IGEPAL® CA-630
0.05% Tween® 20
(pH 8.9 @ 25°C)
Usage Concentration
1.25 units/50μl reactionStorage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C
Heat Inactivation
No5' - 3' Exonuclease
Yes3' - 5' Exonuclease
YesStrand Displacement
NoUnit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNAError Rate
< 140x10-6basesFeatures
- Ideal for routine, AT- and GC-rich PCR
Application Features
- High Sensitivity PCR
- High Throughput PCR
- Routine PCR
- GC-rich PCR
- AT-rich PCR
- Colony PCR
- Long PCR (up to ~6 kb genomic)
Related Products
Companion Products
Product Notes
- The OneTaq High GC Enhancer should not be used alone. It should be added only to reactions with the OneTaq GC Reaction Buffer and will typically improve yields when other conditions have failed.
- Product specifications for individual components in the OneTaq DNA Polymerase mix are available separately.
References
- Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
- Saiki R.K. et al. (1985). Science. 230, 1350-1354.
- Powell, L.M. et al. (1987). Cell. 50, 831-840.
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
Application Notes
Tools & Resources
Selection Charts
Web Tools
FAQs & Troubleshooting
FAQs
- What ends will my PCR products have?
- How should I determine the appropriate annealing temperature for my reaction?
- What should the final primer concentration be in my PCR?
- What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
- What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
- Can Taq/OneTaq® DNA Polymerases be used for nick translation?
- How can I optimize my PCR when using Taq DNA Polymerase?
Troubleshooting
Tech Tips
Citations & Technical Literature
Citations
Additional Citations
- Megan S Thoemmes, Daniel J Fergus, Julie Urban, Michelle Trautwein, Robert R Dunn (2014) Ubiquity and diversity of human-associated demodex mites. PLoS One; 9, e106265. PubMedID: 25162399, DOI: 10.1371/journal.pone.0106265
- Matousková, M., Vesely, P., Daniel, P., Mattiuzzo, G., Hector, R.D., Scobie, L., Takeuchi, Y. and Hejnar, J. (2013) Role of DNA methylation in expression and transmission of porcine endogenous retroviruses. PLoS One; PubMedID: 23986605
- Liang, Y., Basu, D., Pattathil, S., Xu, W.L., Venetos, A., Martin, S.L., Faik, A., Hahn, M.G. and Showalter, A.M. (2013) Biochemical and physiological characterization of fut4 and fut6 mutants defective in arabinogalactan-protein fucosylation in Arabidopsis. PLoS One; PubMedID: 24127514
- Malyshev, D.A., Dhami, K., Quach, H.T., Lavergne, T., Ordoukhanian, P., Torkamani, A. and Romesberg, F.E. (2012) Efficient and sequence-independent replication of DNA containing a third base pair establishes a functional six-letter genetic alphabet. Proc Natl Acad Sci U S A; 109, 4503-4508. PubMedID: 22773812
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M0480S_L_X_v1_0091503
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Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.OneTaq® DNA Polymerase
OneTaq® Standard Reaction Buffer Pack
OneTaq® GC Reaction Buffer Pack
OneTaq® High GC Enhancer
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
Purchase of this product provides the purchaser with a non-exclusive license to use OneTaq® DNA Polymerase for research purposes only.NEW ENGLAND BIOLABS®, ONETAQ® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
DEEP VENT® is a trademark of New England Biolabs, Inc.
IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas, LLC.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.