NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis ModuleProduct information
NEBNext Ultra II Non-Directional RNA Second Strand Module
NEBNext Ultra II Non-Directional RNA Second Strand Module
Applicable for COVID-19 research
Many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this coronavirus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against the COVID-19 pandemic by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support. Are you in SARS-CoV-2 research? Let us know how we can support your research and receive a discounted quotation.
Product IntroductionThis module is part of the non-directional Ultra™ II RNA workflow, for Illumina®- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
Kits are also available that include reagents for every step in the non-directional Ultra II RNA library construction workflow (NEBNext® Ultra II RNA Library Prep Kit for Illumina, NEB #E7770 and NEBNext Ultra II RNA Library Prep with Sample Purification Beads, NEB #E7775).
The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow.
The NEBNExt Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:
- NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) or NEBNext RNA First Strand Synthesis Module (NEB #E7525)
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7442)
- NEBNext Ultra II Ligation Module (NEB #E7595) or NEBNext Ultra Ligation Module (NEB #E7445)
- NEBNext Ultra™ II Q5® Master Mix (NEB #M0544) or NEBNext High Fidelity 2X PCR Master Mix (NEB #M0541)
- NEBNext® Oligos for Illumina® (#E7535, NEB #E7350, NEB #E7335, #E7500, #E7710, #E7730, #E7600, #E6609)
For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact [email protected] for further information.
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext® Second Strand Synthesis Enzyme Mix||-20|
|NEBNext® Second Strand Synthesis Reaction Buffer||-20|
- Product Categories:
- Reverse Transcriptases & RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases Products,
- RNA Library Prep Products
Advantages and Features
- RNA Sample Preparation
- Second Strand Synthesis of cDNA
Properties & Usage
Materials Required but not SuppliedMagnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
Protocols, Manuals & Usage
Usage & Guidelines
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
NEBNext® Second Strand Synthesis Enzyme Mix
NEBNext® Second Strand Synthesis Reaction Buffer
Legal and DisclaimersThis product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
LicensesPhusion® is a registered trademark and property of Thermo Fisher Scientific.
Notice to Customer: Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.
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The supporting documents available for this product can be downloaded below.