Product Class: Other

NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module

Please note: kit name has been edited but the components have not changed.

Product Introduction

This module is part of the non-directional Ultra™ II RNA workflow, for Illumina®- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.

Kits are also available that include reagents for every step in the non-directional Ultra II RNA library construction workflow (NEBNext® Ultra II RNA Library Prep Kit for Illumina, NEB #E7770 and NEBNext Ultra II RNA Library Prep with Sample Purification Beads, NEB #E7775).
Catalog # Size Concentration
E6111S 20.0 reactions
E6111L 100.0 reactions
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Product Information


The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow.

The NEBNExt Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:



Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E6111S     -20    
  • E6111L     -20    
This product is related to the following categories:
Reverse Transcriptases & RT-PCR Products,
cDNA Synthesis & Reverse Transcriptases Products,
RNA Library Prep Products,
This product can be used in the following applications:
NEBNext® Ultra™ II RNA Library Prep,
RNA For Illumina,
Illumina Library Preparation

Advantages and Features

Application Features

  • RNA Sample Preparation
  • Second Strand Synthesis of cDNA

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Protocols, Manuals & Usage


  1. Protocol for NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (E6111)


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.


The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.


Phusion® is a registered trademark and property of Thermo Fisher Scientific.

Notice to Customer: Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.