NEBNext® Ultra™ II Ligation ModuleProduct information
Applicable for COVID-19 research
Many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this coronavirus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against the COVID-19 pandemic by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support. Are you in SARS-CoV-2 research? Let us know how we can support your research and receive a discounted quotation.
Product IntroductionThis module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext® Ultra II End Repair/dA-Tailing Module (NEB #E7546), for Illumina®-compatible library construction.
The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or the NEBNext Ultra II FS DNA Module (NEB #E7810).
The NEBNext Ultra II Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.
The NEBNext Ultra II Ligation Module is Designed for use with the Following:
• NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
• NEBNext Ultra II Q5® Master Mix (NEB #M0544)
• NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, NEB #E7350)
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext® Ligation Enhancer||-20|
|NEBNext® Ultra™ II Ligation Master Mix||-20|
Properties & Usage
Materials Required but not SuppliedMagnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
Protocols, Manuals & Usage
FAQs & Troubleshooting
Citations & Technical Literature
- Resende, P. (2020) Long reads nanopore sequencing to recover SARS-CoV-2 whole genome V.3 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bfy7jpzn
- Quick, J. (2020) nCoV-2019 sequencing protocol v2 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bdp7i5rn
- Oxford Nanopore Technologies (2020) PCR Tiling of COVID-19 Virus Oxford Nanopore Technologies Community; Protocol version: PTC_9096_V109_REVF_06FEB2020;
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
NEBNext® Ligation Enhancer
NEBNext® Ultra™ II Ligation Master Mix
Legal and DisclaimersThis product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
The supporting documents available for this product can be downloaded below.