Product Class: Kit

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

Product Introduction

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) (NEB #7335S/L) includes 12 indices. 

Catalog # Size Concentration
E7335S 24.0 reactions (12.0 indices)
E7335L 96.0 reactions (12.0 indices)

Protocols, Manuals & Usage

Protocols

  1. Refer to the manual (NEB #E7335) for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Are NEBNext adaptors and primers for Illumina validated in Next Generation Sequencing Workflows?
  2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
  3. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
  4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
  5. Are these reagents available in a customized format, larger format or 96 well formats?
  6. Can USER Enzyme be purchased separately?
  7. Are the NEBNext Oligos for Illumina compatible with single read and paired end sequencing?
  8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
  9. How do I know the concentration of my index primers? 
  10. Do you have a Sample Sheet with the index sequences that can be used in the Illumina Experiment Manager (IEM)?
  11. How do I use the NEB provided Sample Sheet for my specific instrument?
  12. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

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