Product Class:

NEBNext® Ultra™ II End Repair/dA-Tailing Module

Product Introduction

This module is part of the Ultra II workflow, and is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction. 

This module is also compatible with some Oxford Nanopore Technologies® workflows, including some involving COVID-19 research.

Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103).

Bioz Badge Exists : False
Catalog # Size Concentration
E7546S 24 reactions 0 %
E7546L 96 reactions 0 %

Product Information

Description


The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5′ phosphorylated, 3′ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.

This module is compatible with Illumina workflows and with several Oxford Nanopore Technologies workflows.

For Illumina library construction, the NEBNext Ultra II End Repair/dA-Tailing Module is designed for use with the following:

• NEBNext Ultra II Ligation Module (NEB #E7595)
• NEBNext Ultra II Q5® Master Mix (NEB #M0544)
• NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, #E7350)

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA Library on the Illumina Sequencing Platform,

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
NEBNext Ultra II End Prep Enzyme Mix -20
NEBNext Ultra II End Prep Reaction Buffer -20
Product Categories:
ChIP-seq Library Prep Products,
DNA Library Prep Products
Applications:
NEBNext® Ultra™ II DNA Library Prep,
DNA Library Preparation,
NGS Sample Prep & Target Enrichment
,
Illumina Library Preparation

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Storage Temperature

-20°C

Protocols, Manuals & Usage

Protocols

  1. Protocol for use with NEBNext® Ultra™ II End Repair/dA-Tailing Module (E7546)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. There are 3 NEBNext Ligation modules, the NEBNext Ultra II Ligation Module (#E7595), the NEBNext Ultra Ligation Module (#E7445) and the NEBNext Quick Ligation Module(#E6056). Which module should be used in conjuction with the NEBNext Ultra II End Repair/dA-Tailing Module?
  2. How do I clean up the reaction once complete?
  3. Can I store the reaction after it is finished in the thermocycler (ie when it reaches the 4°C hold)?
  4. What do I do if I see a precipitate in the Ultra II End Prep Reaction Buffer?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.