NEBNext® Ultra™ II End Repair/dA-Tailing ModuleProduct information
NEBNext Ultra II End Repair/dA-Tailing Module
NEBNext Ultra II End Repair/dA-Tailing Module
Applicable for COVID-19 research
Many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this coronavirus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against the COVID-19 pandemic by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support. Are you in SARS-CoV-2 research? Let us know how we can support your research and receive a discounted quotation.
This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore MinION™workflows.
Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103).
The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5′ phosphorylated, 3′ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.
This module is compatible with Illumina workflows and with some Oxford Nanopore MinION™workflows.
For Illumina library construction, the NEBNext Ultra II End Repair/dA-Tailing Module is designed for use with the following:
• NEBNext Ultra II Ligation Module (NEB #E7595)
• NEBNext Ultra II Q5® Master Mix (NEB #M0544)
• NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, #E7350)
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on aEach set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA Library on the Illumina Sequencing Platform,
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext Ultra II End Prep Enzyme Mix||-20|
|NEBNext Ultra II End Prep Reaction Buffer||-20|
Properties & Usage
Materials Required but not SuppliedMagnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
Protocols, Manuals & Usage
FAQs & Troubleshooting
- There are 3 NEBNext Ligation modules, the NEBNext Ultra II Ligation Module (#E7595), the NEBNext Ultra Ligation Module (#E7445) and the NEBNext Quick Ligation Module(#E6056). Which module should be used in conjuction with the NEBNext Ultra II End Repair/dA-Tailing Module?
- How do I clean up the reaction once complete?
- Can I store the reaction after it is finished in the thermocycler (ie when it reaches the 4°C hold)?
Citations & Technical Literature
- Resende, P. (2020) Long reads nanopore sequencing to recover SARS-CoV-2 whole genome V.3 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bfy7jpzn
- Quick, J. (2020) nCoV-2019 sequencing protocol v2 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bdp7i5rn
- Oxford Nanopore Technologies (2020) PCR Tiling of COVID-19 Virus Oxford Nanopore Technologies Community; Protocol version: PTC_9096_V109_REVF_06FEB2020;
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
NEBNext Ultra II End Prep Enzyme Mix
NEBNext Ultra II End Prep Reaction Buffer
Legal and DisclaimersThis product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
The supporting documents available for this product can be downloaded below.