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High throughput DNA & Chromatin shearing

The Q800R3 is the 2nd generation DNA and Chromatin shearing system. Improvements include an enhanced user-friendly design and quieter operation while remaining thousands of dollars less than the competition.

Samples can be sheared to a range of fragment sizes (150bp – 3kb). The system is compatible with commercially available sample tubes and is capable of processing up to 18 samples at one time. Multiple tube rack options can accommodate 50ul - 1ml sample volumes using a variety of standard sample tubes.

The Q800R3 is a complete package including a chiller to control temperature, digital operating system with programmable memory, high intensity ultrasonics and sound reducing enclosure.

Principles

The ultrasonic electronic generator transforms AC line power to a 20 KHz signal that drives a piezoelectric converter/transducer. This electrical signal is converted by the transducer to a mechanical vibration due to the characteristics of the internal piezoelectric crystals.

The vibration is amplified and transmitted down the length of the titanium horn where the surface longitudinally expands and contracts. The distance that the face of the horn travels is dependent on the amplitude selected by the user through the keypad. As you increase the amplitude setting the excursion of the horn’s face will increase and sonication intensity will also increase within your liquid sample.

In liquid, the rapid vibration of the horn surface causes cavitation, the formation and violent collapse of microscopic bubbles. The collapse of thousands of cavitation bubbles releases tremendous energy in the cavitation field. In the Q800 system, the cavitation travels through the water and the wall of each sample tube, processing the liquid inside the tube.

Find out why customers like NIH, Harvard and Genentech have adopted our technology for use with multiple NGS platforms and see the new Illumina^® technical note highlighting the Q800R3 system.

System Specifications:

The Q800R3 includes:

• Generator
• Converter
• Ultrasonic Horn
• Rotation System
• Chiller
• Cables
• Tubing
• 3 tube rack options
• 2-year warranty

Applications:

• DNA shearing for Next-gen sequencing
• Chromatin shearing
• ChIP
• ChIP-seq
• RNA-seq
• Protein extraction
• Cell lysis

Key Installations:

• Broad Institute
• NIH
• Harvard Medical School
• Broad Institute
• Memorial Sloan Kettering
• Max Planck Institute
• Ludwig Institute UCSD
• University of Cambridge
• Washington Univ. St. Louis
• California Institute of Technology
• Shanghai Inst. for Biological Sciences

For more information, to request a quote or demonstration: contact our technical support team