Product Class: Nucleic Acid Marker

Fast DNA Ladder

Suitable for 50-200 gel lanes
Catalog #SizeConcentrationGel Lanes
N3238S1 ml25 μg/ml50

Description

The Fast DNA ladder is a pre-mixed, ready-to-load molecular weight marker containing xylene cyanol FF dye as a tracking dye. 

The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for fast electrophoresis systems as well as standard electrophoresis. The digested DNA includes fragments ranging from 50 base pairs to 10 kilobases. The 1 kb fragment has increased intensity to serve as reference band.

N3238_thumb

Fast DNA Ladder. Mass values are for 0.5 μg/lane

Properties and Usage

Bases

FragmentMassbp
13610,002
2365,001
3363,001
4382,000
5281,500
61081,000
743766
840500
933300
1041150
116150

Effective Size Range

50bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

0.002% Xylene Cyanol
5 mM Tris-HCl
5 mM EDTA
5% Glycerol
pH 8.0 @ 25°C

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. Preparation: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. Usage Recommendation: For standard electrophoresis applications, we recommend loading 20 µl (0.5 µg) of the Fast DNA Ladder per gel lane. The best separation occurs on a 1.2% agarose gel. Below 1%, the 50 bp fragment may not separate from the 150 bp fragment. For use on a fast electrophoresis system, follow the loading recommendations of the system’s manufacturer (5 to 20 µl). The Fast DNA Ladder is suitable for use on the FlashGel® System from Lonza (1.2% gel) or on the E-Gel® system from Invitrogen (1.2 or 2% gels). The Fast DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  3. Fast DNA Ladder is stable for at least 6 months at 25°C.
  4. For long term storage. Store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor(Ed.), Molecular Cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor Laboratory Press.
  1. How much Fast DNA Ladder should I load on a gel?
  2. What percentage of agarose gel should I use?
  3. Can I store it at -20°C?
  4. Can I use GelRed with the DNA Ladders from NEB?
  5. Can I use SYBR® with the DNA Ladders from NEB?