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Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeats) immune system has been adapted for genome-scale screening by combining Cas9 with pooled guide RNA libraries. Here we describe a protocol for genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system.
Read about the advantage of using NucleoBond Endotoxin Free Plasmid DNA from MACHEREY-NAGEL to avoid endotoxicity in virus production and mammalian cell culture.
Struggling with DNA isolation from hard-to-lyse samples? Up to 60% more DNA compared to standard extraction methods.
An accurate and cost-effective total workflow solution for the characterization of EGFR in FFPE material.
This application note presents data showing the performance of the RainDrop Digital PCR system on FFPE DNA and low input cell free DNA (cfDNA) using the ThunderBolts Cancer Panel.
This application note describes how to assembly multiple fragments and have 100% correct clones.
The RainDrop system lower limit of detection for the KRAS p.G12D (c.35 G>A) mutation is 1/45,000 (3.5E+07 WT copies examined). This highly sensitive and specific method has the potential to be employed in the clinic, including diagnosis, cancer recurrence monitoring, and treatment management.
After nearly 40 years of offering restriction enzymes to the research community, New England Biolabs (NEB) has earned the reputation of being a leader in enzyme technologies. NEB offers scientists 276 restriction enzymes, the largest selection in the industry.
A PhD student working at the Department of Immunology at the University Medical Center Utrecht shares her positive experience with the NucleoBond Xtra Midi Kit from MACHEREY-NAGEL
Visualization of Wnt receptor internalization by SNAP-tag technology from New England Biolabs (NEB)
The epigenetic status of genes can be assayed by chromatin immunoprecipitation (ChIP). ChIP is a powerful technique to study DNA-protein interactions. To explore the genome-wide binding of transcription factors and the epigenetic landscape ChIP can be combined with sequencing (ChIPseq). This technique enables exploration of the epigenetic state in a global fashion
In this application note we describe the use of the Gibson Assembly™ Master Mix from New England BioLabs (NEB) together with gBlocks™ Gene Fragments from Integrated DNA Technologies (1) for the creation of a basic cloning vector for IP3R research.
Validating an antibody for immunohistochemistry (IHC) use involves more than just observing a signal in cells.
Marjorie Mercier, a scientist in Brussels, shares her positive experience with Q5 High-Fidelity DNA Polymerase for the amplification of the IgM variable region.
Colette Duez of the Center of Protein Engineering/ULg shares her positive experience with Q5 High-Fidelity DNA Polymerase for the amplification of a GC-rich (73%) insert of 1.4 kb from bacterial origin.
Mrs. Evy Vanderheyden, working at the VIB/KULeuven Molecular Microbiology and Biotechnology department shares her positive experience with Q5 High-Fidelity DNA Polymerase for the robust amplification of genomic yeast DNA.
Improved methods for site-directed mutagenesis using Gibson Assembly Master Mix from New England Bioloabs
SimpleChIP® in Literature: A synopsis of a recent publication by Tomas J. Bos, Ph.D., Department of Hematology and Immunology, Vrije Universiteit Brussel, Brussels, Belgium
BIOKÉ is offering multiple solutions to improve your next generation sequencing sample prep and data-analysis.