Product Class: Other

DNA Polymerase I (E. coli)

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Product Introduction

DNA Polymerase I (E. coli) is a DNA-dependent DNA polymerase with inherent 3′– 5´ and 5′– 3′ exonuclease activities

  • Nick translation of DNA
  • Second strand cDNA synthesis
Catalog # Size Concentration
M0209S 500 units 10,000 units/ml
M0209L 2,500 units 10,000 units/ml

Faqs & Tech Tips

FAQs

  1. Can DNA Polymerase I (E. coli) be used in other NEBuffers, including CutSmart?
  2. Can DNA Polymerase I (E. coli) be used to blunt DNA?
  3. Can DNA Polymerase I (E. coli) be used to fill in 3' overhangs?
  4. Why are there artifacts in my blots using the nick translated probe?
  5. Can DNA Polymerase I (E. coli) be used to remove 5' overhangs?
  6. Can DNA Polymerase I (E. coli) be heat inactivated?
  7. Can DNA Polymerase I (E. coli) be used in nick translation protocols?
  8. Is nick translation the best way to make a labeled probe?
  9. Are NEB DNA Polymerases supplied with dNTPs?

Protocols & Manuals

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Selection Charts

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.