Product Class: Other

DNase I (RNase-free)

icon_cloned icon_recomb icon_NEBU icon_incTemp37 icon_HI_75

Product Introduction

  • Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends
  • Degrades DNA template in transcription reactions
  • Removes contaminating genomic DNA from RNA samples
  • Isolated from a recombinant source
Catalog # Size Concentration
M0303L 5,000 units 2,000 units/ml
M0303S 1,000 units 2,000 units/ml

Product Information

Description

Highlights

Isolated from a recombinant source
Supplied with 10X Reaction Buffer DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Product Source

An E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
DNase I Reaction Buffer -20 10 X
Product Categories:
RNA Synthesis Products,
Nuclease Products

Advantages and Features

Application Features

  • Degradation of DNA template in transcription reactions
  • Removal of contaminating genomic DNA from RNA samples
  • DNase I footprinting
  • Nick Translation

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.

Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

Reaction Conditions

1X DNase I Reaction Buffer
Incubate at 37°C

1X DNase I Reaction Buffer
10 mM Tris-HCl
2.5 mM MgCl2
0.5 mM CaCl2
(pH 7.6 @ 25°C)

Usage Concentration

2,000 - units/ml

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
2 mM CaCl2
50% Glycerol
pH 7.6 @ 25°C

Heat Inactivation

75°C for 10 min

Notes

  1. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3).

References

  1. Kunitz, M. (1950). J. Gen. Physiol . 33, 349-362.
  2. Vanecko, S. and laskowski, M. (1961). J. Biol. Chem . 236, 3312-3316.
  3. Huang, Z. et al. (1996). Biotechniques . 20, 1012-1020.

Faqs & Tech Tips

FAQs

  1. What is the specific activity of DNase I(RNase-free)?
  2. Will DNase I work in NEB buffers 1-4?
  3. What is the best way to remove DNase I from my reaction?
  4. Will DNase I work in CutSmart buffer?

Protocols & Manuals

Protocols

  1. A Typical DNase I Reaction Protocol (M0303)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

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Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.