DNase I (RNase-free)Product information
HighlightsIsolated from a recombinant source
Supplied with 10X Reaction Buffer DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.
Product SourceAn E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|DNase I Reaction Buffer||-20||10X|
Advantages and Features
- Degradation of DNA template in transcription reactions
- Removal of contaminating genomic DNA from RNA samples
- DNase I footprinting
- Nick Translation
Properties and Usage
Unit DefinitionOne unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.
Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.
1X DNase I Reaction Buffer
Incubate at 37°C
1X DNase I Reaction Buffer:
10 mM Tris-HCl
2.5 mM MgCl2
0.5 mM CaCl2
pH 7.6 @ 25°C
10 mM Tris-HCl
2 mM CaCl2
pH 7.6 @ 25°C
Heat Inactivation75°C for 10 min
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
- RNase Activity (2 Hour Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 2 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DNase I (RNase-free)
DNase I Reaction Buffer
- EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3).
- Kunitz, M. (1950). J. Gen. Physiol . 33, 349-362.
- Vanecko, S. and laskowski, M. (1961). J. Biol. Chem . 236, 3312-3316.
- Huang, Z. et al. (1996). Biotechniques . 20, 1012-1020.