NEBuffer™ 4Product information
NEBuffer Pack #4 (green)
|5 ml ( 10 X )||-||Unavailable in your region|
Product IntroductionNew England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity.
This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA.
For the BSA containing formulation (which is included with NEB restriction enzymes), select CutSmart® Buffer (NEB #B7204).
DescriptionNew England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1 (NEB #B7201), NEBuffer 3.1 (NEB #B7203) and CutSmart Buffer (NEB #B7204) are available. Visit www.neb.com for details.
- Restriction Enzyme Digestion
Properties and Usage
1X Master Mix Composition
50 mM Potassium AcetatepH 7.9 @ -20°C20 mM Tris-acetatepH 7.9 @ -20°C10 mM Magnesium AcetatepH 7.9 @ -20°C1 mM DTTpH 7.9 @ -20°C
1X Master Mix Composition
50mM Potassium Acetate (pH 0 @ 0°C)pH 0 @0°C20mM Tris-acetate (pH 0 @ 0°C)pH 0 @0°C10mM Magnesium Acetate (pH 0 @ 0°C)pH 0 @0°C1mM DTT (pH 0 @ 0°C)pH 0 @0°C
Faqs & Tech Tips
- What is supplied with the NEBuffer 4 pack?
- What is the composition of NEBuffer 4?
- How should the NEBuffer be used?
- The buffer arrived thawed. Are the buffer and the enzyme still active?
- Why is my Restriction Enzyme not cutting DNA?
- Why do I see additional DNA bands on my gel after a restriction digest?
- Why do I see a DNA smear on an agarose gel after a restriction digest?
- How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
Protocols & Manuals
Other Tools & Resources
Quality & Safety
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
The supporting documents available for this product can be downloaded below.