Q5 High-Fidelity DNA Polymerase

 
Q5® High-Fidelity DNA Polymerase from New England Biolabs (NEB) sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (>100 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
 
Read more about the different formats of this high-fidelity enzyme:
 

Q5 High-Fidelity DNA Polymerase

Q5 High-Fidelity 2X Master Mix

Q5 Hot Start High-Fidelity DNA Polymerase

Q5 Hot Start High-Fidelity 2X Master Mix

Sample request Q5 High-Fidelity DNA Polymerase

 
See what our customers are saying about Q5® High-Fidelity DNA Polymerases. Do you want to test yourself? Fill in your details below and receive a Q5 DNA Polymerase sample**. 
 

**This free sample can be requested by laboratory from the Netherlands, Belgium and Luxembourg until June 30, 2017.  No cash or cash equivalent. Offer may not be applied to existing, pending or prior orders. Free samples cannot be requests by current users

Q5® High-Fidelity DNA Polymerases Feedback

"I have started using Q5 for single cell PCR and it is working brilliantly. I get very bright bands and am able to derive sequences from nearly all the positive wells." - Postdoc, King’s College London

"My Q5 sample arrived just as I was struggling with what I had anticipated would be a routine Phusion reaction. After a couple of failures with Phusion, I tried the Q5 sample, head-to-head, under a few different reaction conditions. All of my Q5 reactions worked beautifully, while all of the Phusion reactions failed. After that observation, I repeated a recent successful Phusion reaction with both Phusion and Q5 to compare the products in circumstances under which Phusion worked very well. In that set of tests, all reactions were successful, but the crude gels showed more product in each Q5 reaction and clearer, cleaner bands. Since Q5 is priced competitively compared to Phusion, we have started replacing Phusion with Q5 in our standard reactions. So far, we have not seen any reactions work with Phusion and not with Q5. We hope this will continue to be the case! I have started recommending Q5 to other scientists who are faced with tricky PCR reactions. Two months ago, I would've recommended Phusion, but now it's Q5." - Lab Manager, Colorado State University

"Q5 High Fidelity DNA Polymerase from NEB is a superb product. I am currently using this to amplify some of my DNA inserts and have repeatedly come up with very positive results better than what I got using another brand not in any way associated with NEB. Am I surprised by the result I am getting from Q5? Absolutely not! Any bioscience kit with NEB on it must be good."
Researcher
University of North Texas

"Q5® works great. It was able to amplify a very difficult product, one I honestly didn't think would work. I am extremely happy with the Q5 enzyme." - Research Asst., Vanderbilt University

"We switched from KOD to phusion polymerase which at the time seemed the best. But now, the new Q5 polymerase is among the best available in the market, even better than phusion. Highly recommended to others!" - Ph.D. Student, University of Cambridge

"100% satisfaction: very specific and vast synthesis of the PCR product on BAC-clone template." - Research Associate, Fordham University

"Not only did I observe great amplification of my product but there were no contaminating bands and the desired amplicon was as tight as any I have ever seen." - Research Assistant, Colorado State University

"We work on an organism with a high GC content and the gene I was amplifying is 70% GC. The 1.9kb band was gorgeous and I got nothing from Phusion. I love it. It will be our new lab polymerase." - Professor, Washington University School of Medicine

"Q5 DNA Polymerase gave me wonderful results on the first shot." - Research technologist, University of Nebraska Medical Center

"The Q5 works great, way better than the Pfu Turbo I was using. I have already ordered Q5 polymerase for my experiments." - Postdoc, MIT

"I have a 75% GC rich region that I need to amplify and tried all possible polymerases one can think of. Nothing worked. With Q5 I was so happily surprised when I looked at the gel and saw single bands of the right size!" - Customer, NIBR