Product Class: Kit

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

For use in conjunction with this kit, the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) now has an Express protocol option for substantial time savings and a more streamlined workflow.

Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765).

Product Introduction

Even more from less, for RNA

Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of strand-specific RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries and enabling use of lower input amounts and fewer PCR cycles. The Ultra II Directional RNA kit uses the “dUTP method” (1, 2) for strand-specificity, and has a streamlined, automatable workflow.


Catalog # Size Concentration
E7760S 24.0 reactions
E7760L 96.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Where can I find protocols for using NEBNext® RNA Library Prep reagents?

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

FAQs & Troubleshooting

FAQs

  1. I have USER enzyme from my library prep kit (blue cap) and USER enzyme from my NEBNext Index Primer kit (red cap). Are they the same?
  2. What is the difference between the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) and the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770)?
  3. What is the difference between the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) and the original NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420)?
  4. What is the starting material I need to use when preparing libraries using the NEBNext Ultra II Directional RNA kit?
  5. Which kit can I use to isolate Poly (A) mRNA from Total RNA?
  6. Which kit can I use to deplete ribosomal RNA (rRNA) from human, mouse or rat RNA?
  7. Does the kit include adaptor and primers?
  8. Is it possible to reduce the volume of beads added during the second strand synthesis cleanup step of the protocol for E7760, E7765, E7770, and E7775?
  9. Which NEBNext Oligos can be used with this library prep kit?
  10. Can I use this NEBNext kit with adaptors and primers from other vendors than NEB?
  11. What do I do if I see a precipitate in the Ultra II End Prep Reaction Buffer?

Troubleshooting