Product Class: Kit

NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

Product Introduction

Globin mRNA and rRNA are highly abundant in blood samples, and their efficient and specific removal is desirable in order to reveal the biological significance of less abundant transcripts.

The NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete globin mRNA, cytoplasmic rRNA and mitochondrial rRNA with human, mouse and rat samples, and is effective with intact and degraded RNA.

  • Efficient, specific depletion of rRNA and globin mRNA (adult, fetal and embryonic)
  • Suitable for low-quality or high-quality RNA
  • Compatible with a broad range of input amounts: 10 ng - 1 μg
  • Optional integration with poly(A) mRNA isolation workflows for removal of globin RNAs, rRNAs, and noncoding RNAs
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available without RNAClean® beads.

Catalog # Size Concentration
E7755S 6.0 reactions
E7755X 96.0 reactions

Protocols, Manuals & Usage

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What species can I use the NEBNext® Globin mRNA & rRNA Depletion Kit for? Will it work for other species?
  2. Which globin mRNAs and rRNAs are depleted with the NEBNext® Globin mRNA & rRNA Depletion Kit (Human/Mouse/Rat)? 
  3. Can I use the NEBNext® Globin mRNA & rRNA Depletion Kit (Human/Mouse/Rat) with degraded RNA or fragmented RNA?
  4. What is the total RNA input I should use?
  5. Why must the RNA be free of DNA?
  6. What is the expected RNA yield recovered after globin mRNA and rRNA depletion?
  7. What is the percentage of globin mRNA and rRNA left after depletion?
  8. How can I determine if the globin mRNA and rRNA depletion was efficient?
  9. I am only interested in coding RNA. Can I use the NEBNext® Globin mRNA & rRNA Depletion Kit (Human/Mouse/Rat) to remove globin mRNA from previously enriched mRNA.
  10. Does the NEBNext® Globin mRNA & rRNA Depletion Kit (Human/Mouse/Rat) work for ribosomal footprinting or profiling applications?
  11. Can the enriched RNA resulting from the NEBNext depletion kit be used in long read sequencing applications?

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