R0585 FAQs for BlpI, Restriction Endonucleases, Intl, NEB
New England Biolabs
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BlpI FAQ

Q1: Do degenerate recognition sites need to be palindromic?
Q2: How can this enzyme be inactivated?

Q1: Do degenerate recognition sites need to be palindromic?

A1: Most restriction enzyme recognition sites are palindromic and include only specified base pairs (i.e., EcoRI recognizes GAATTC). However, some enzymes have degenerate sites, meaning that they contain one or more base pairs that are not specifically defined (i.e., BsrFI recognizes RCCGGY, where R= A or G and Y= C or T). For degenerate enzymes, any base represented by the single letter code may be present at either location in the recognition site for cleavage to occur. For example, BsrFI recognizes all of the following sequences: ACCGGC, ACCGGT, GCCGGC, GCCGGT.


Q2: How can this enzyme be inactivated?

A2: To inactivate enzymes that cannot be heat killed, we recommend a phenol extraction followed by ethanol precipitation or a commercial spin column designed to purify DNA.