Product Class: Restriction Endonuclease

NotI
neb31 cloned at NEB recombinant time saver dil_C 37 65 Heat CpG

NotI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10232248. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GC^GGCC_GC

Product Introduction

Catalog # Size Concentration
R0189S 500.0 units 10000 units/ml
R0189L 2500.0 units 10000 units/ml
R0189M 2500.0 units 50000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  2. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  3. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  4. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting