Product Class: Other

Sulfolobus DNA Polymerase IV
NEBU cloned at NEB recombinant 55 No

The L size of this product has been discontinued.  The S size will still be available.

Product Introduction

  • Sulfolobus DNA Polymerase IV is a thermostable Y-family lesion-bypass DNA Polymerase
  • Efficiently synthesizes DNA across a variety of DNA template lesions
  • Can be used for DNA repair
Catalog # Size Concentration
M0327S 100.0 units 2000 units/ml

Product Information

Description

 

Product Source

An E. coli strain that carries the gene encoding DNA polymerase IV from Sulfolobus islandicus.
This product is related to the following categories:
DNA Manipulation Products
This product can be used in the following applications:
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 55°C.

Reaction Conditions

1X ThermoPol® Reaction Buffer
Incubate at 55°C

1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 40000 daltons

5' - 3' Exonuclease

No

3' - 5' Exonuclease

No

Strand Displacement

No

Unit Assay Conditions

1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13mp18.

Activity at Temperature

@30°C: 5%
@37°C: 20%
@45°C: 40%
@65°C: 90%
@72°C: 70%

Application Features

  • Synthesis of DNA through DNA lesions (1,2)
  • Repair of DNA (1)

References

  1. Boudsocq, F. et al. (2001). NucleicAcids Res.,. 29, 4607-4616.
  2. McDonald, J.P. et al. (2006). NucleicAcids Res.,. 34, 1102-1111.

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

THERMOPOL® is a registered trademark of New England Biolabs, Inc.

TRITON® is a registered trademark of Union Carbide Corporation.

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