Product Class: Other

T7 RNA Polymerase
NEBU recombinant 37

GMP-grade reagent also available. Learn more.

Product Introduction

T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. Applications include:

Catalog # Size Concentration
M0251S 5000.0 units 50000 units/ml
M0251L 25000.0 units 50000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Protocol for Standard RNA Synthesis

Application Notes

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What is the promoter sequence of T7 RNA Polymerase?
  2. Is it possible to start transcription with an A?
  3. Does the transcription reaction with T7 RNA Polymerase require a primer?
  4. Does T7 RNA Polymerase leave an extra base at the end of a transcript?
  5. Will T7 RNA Polymerase work on single stranded substrate?
  6. Will T7 RNA Polymerase work on uncut plasmid DNA?
  7. Can aberrant RNA be produced when using T7 RNA Polymerase?
  8. How can the yield of RNA be maximized when using T7 RNA Polymerase?
  9. Can I use T7 RNA Polymerase to make high specific activity radiolabeled probes?
  10. What are the main causes of reaction failure using T7 RNA Polymerase?
  11. Why is the specific activity of the probe low?
  12. How can the yield of RNA be maximized when using T7 RNA Polymerase?