ssRNA LadderProduct information
|25 gel lanes ( 2000 µg/ml )||-||Unavailable in your region|
DescriptionThe ssRNA Ladder is a set of 7 RNA molecules produced by in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000, 1000 and 500 bases. The 3000 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing or native agarose gels.
This marker was not designed for precise quantification of ssRNA mass.
Denaturing vs. Native Agarose Gels
It is common practice to electrophorese RNA on a fully denaturing agarose gel, such as one containing formaldehyde (1). However, in many cases it is possible to run RNA on a native agarose gel and obtain suitable results. In fact, it has been demonstrated that treatment of RNA samples in a denaturing buffer maintains the RNA molecules in a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarose gels eliminates (see other side) problems associated with toxic chemicals and the difficulties encountered when staining and blotting formaldehyde gels.
The ssRNA Ladder is also compatible with formaldehyde-based loading buffers.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|RNA Loading Dye, (2X)||2X|
Properties and Usage
20 mM sodium citrate
1 mM EDTA
pH 6.0 @ 25°C
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
RNA Loading Dye, (2X)
- Minimize repeated freeze-thaw cycles. It is best to aliquot the marker into single use portions.
- To avoid ribonuclease contamination: wear gloves, use RNase-free water for gels and buffers, wash equipment with detergent and rinse thoroughly with RNase-free water.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.).
- Liu, Y.-C. and Chou, Y.-C. (1990). Biotechniques. 9
- Cook, S. and Marchetti, C. Unpublished observation
- I need to know what types of gel to use to run RNA ladders?
- What types of gel and staining dyes can I use for NEB’s ssRNA Ladder (N0362S)?
- How much of the ssRNA Ladder do I need to load on a FlashGel 1.2% (Lonza)?
- Can I run the ssRNA Ladder on a formaldehyde gel, and if so, how much of the ladder should I use?