DescriptionThe RNA Loading Dye, (2X) is a premixed loading dye for use with denaturing and non-denaturing PAGE/agarose gels.
0.01% bromophenol blue
0.005% Xylene Cyanol
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 16 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
- RNase Activity (16 Hour Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 16 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.
Material Safety Datasheets
- RNA Loading Dye, (2X) is conveniently supplied in 4 tubes. The dye can be stored at room temperature for a week, at 4°C for a month and at -20°C for 2 years.
- The dye can also be used as a stop solution for enzyme reactions. After mixing, the samples can be stored at -20°C for at least 3 days before gel analysis.