Product Pathways - MAPK Signaling
Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb #5841
|5841S||100 µl (10 western blots)||---||In Stock||---|
|5841P||40 µl (4 western blots)||---||In Stock||---|
|5841||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||40||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, ChIP=Chromatin IP
Species predicted to react based on 100% sequence homology: Monkey, Bovine, Horse.
Specificity / Sensitivity
Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb recognizes endogenous levels of FRA1 protein only when phosphorylated at Ser265. This antibody may also cross-react with phospho-FRA2, but does not cross-react with phospho-c-Fos or phospho-FosB.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser265 of human FRA1 protein.
Western blot analysis of extracts from HeLa cells, serum-starved overnight, and either left untreated or treated with TPA #4174 for 4 hours, using Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb #5841 (upper) and FRA1 (D80B4) Rabbit mAb #5281 (lower). Antibody phospho-specificity is shown by treating lysates with λ phosphatase.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).
- Tulchinsky, E. (2000) Histol Histopathol 15, 921-8.
- Dobrazanski, P. et al. (1991) Mol Cell Biol 11, 5470-8.
- Nakabeppu, Y. and Nathans, D. (1991) Cell 64, 751-9.
- Rosenberger, S.F. et al. (1999) J Biol Chem 274, 1124-30.
- Sasaki, T. et al. (2006) Mol Cell 24, 63-75.
- Basbous, J. et al. (2007) Mol Cell Biol 27, 3936-50.
- Kovary, K. and Bravo, R. (1991) Mol Cell Biol 11, 2451-9.
- Kovary, K. and Bravo, R. (1992) Mol Cell Biol 12, 5015-23.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7017 6-Tube Magnetic Separation Rack
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 9007 ChIP-Grade Protein G Agarose Beads
- 9006 ChIP-Grade Protein G Magnetic Beads
- 3372 Erk5 Antibody
- 5281 FRA1 (D80B4) Rabbit mAb
- 2251 FosB (5G4) Rabbit mAb
- 2263 FosB Antibody
- 3371 Phospho-Erk5 (Thr218/Tyr220) Antibody
- 3880 Phospho-FRA1 (Ser265) Antibody
- 4377 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9004 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
- 9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
- 7983 SimpleChIP® Rat CCRN4L Promoter Primers
- 7964 SimpleChIP® Rat GAPDH Promoter Primers
- 2250 c-Fos (9F6) Rabbit mAb
- 4384 c-Fos Antibody
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.