Product Class: Other

Monarch® RNase A

Product Introduction

Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types. Treatment with RNase A is an optional step in the protocol to remove any residual RNA present. This high-quality enzyme preparation is purified from bovine pancreas and is DNase, protease and DNA-free. It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-HCl, pH 7.4.

  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.

Catalog # Size Concentration
T3018L 1.0 ml 20 mg/ml

Product Information

Description

Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types. Monarch RNase A degrades single-stranded RNA at C and U residues. This formulation is purified from cow pancreas, is free of contaminating protease or DNase activity, and contains no residual host DNA. It is used at 56°C in the Monarch Genomic DNA Purification Kit protocol but is active at any temperature between 20°C and 65°C.  It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4.

Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of  any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low; however, an RNase A treatment step is included in each protocol as an option for the user. 
This product is related to the following categories:
RNases,
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

50 mM Tris-HCl
50% Glycerol
pH 7.4

Protocols, Manuals & Usage

Protocols

  1. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
  2. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
  3. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
  4. Genomic DNA Purification from Yeast (NEB #T3010)
  5. Genomic DNA Purification from Saliva (NEB #T3010)
  6. Protocol for Genomic DNA Cleanup (NEB #T3010)
  7. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
  8. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
  9. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
  10. Quick Protocol for Extraction and Purification of Genomic DNA

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Can I use a different RNase A with the Monarch® Genomic DNA Purification Kit? 
  2. Can I omit the RNase A digestion step from the protocol? 
  3. Can I use Monarch® RNase A for applications/workflows other than gDNA purification?
  4. The enzymes in the kit were not stored at -20°C right away. Will they still work?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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