Product Class: Other

Monarch® gDNA Elution Buffer

Product Introduction

The Monarch gDNA Elution Buffer a component of the Monarch Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA  from a variety of sample types. Monarch gDNA Elution Buffer is used for the final elution step, and is suitable for long term storage of gDNA. 

 All Monarch kit components are available for purchase separately, for additional convenience, and to minimize waste.
Catalog # Size Concentration
T3016L 34.0 ml

Product Information

Description

Monarch gDNA Elution Buffer is supplied in 10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA and is suitable for long term storage of gDNA. Nucleases are inactivated both by the inclusion of EDTA and the pH of the solution.
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Quick Protocol for Extraction and Purification of Genomic DNA
  2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
  3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
  4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
  5. Protocol for Genomic DNA Cleanup (NEB #T3010)
  6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
  7. Genomic DNA Purification from Yeast (NEB #T3010)
  8. Genomic DNA Purification from Saliva (NEB #T3010)
  9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
  10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
  2. What is the minimum elution volume that can be used with the Monarch® Genomic DNA Purification Kit columns?
  3. How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
  4. Can I get better recovery with the Monarch® Genomic DNA Purification Kit if I do a second elution with my eluent from the first elution?
  5. Can I use a different elution buffer than the one supplied with the Monarch Genomic DNA Purification Kit (NEB #T3010)?
  6. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
     
  7. How can I assess gDNA integrity and purity?
  8. Can I omit the low-speed binding centrifugation step to save time?
  9. How should I store purified gDNA samples?
  10. When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
  11. Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective May 9, 2022: Shelf life is 24 months.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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