Monarch® Spin High-Capacity DNA Cleanup Kit (100 μg)
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
T1135V |
Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) |
10 preps | - | Unavailable in your region | |
T1135S |
Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) |
50 preps | - | Unavailable in your region | |
T1135L |
Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) |
200 preps | - | Unavailable in your region |
Monarch® Spin High-Capacity DNA Cleanup Kit (100 μg)
Product Introduction
- High-capacity DNA cleanup kit, featuring new precision-engineered spin columns for high performance
- Purify up to 100 μg of DNA. Elute in just 50 μl for highly concentrated DNA
- Unique column design compatible with microcentrifuges and vacuum manifolds helps prevent buffer retention and salt carryover
- No pH monitoring required
- Modified protocol provided for oligonucleotide, genomic DNA, or RCA products, enabling efficient purification of various DNA samples
- Eco-friendly design with significantly less plastic in columns and kit compared to leading suppliers
Catalog # | Size | Concentration |
---|---|---|
T1135V | 10 preps | |
T1135S | 50 preps | |
T1135L | 200 preps |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
The Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) offers class-leading binding capacity and proven Monarch buffer chemistry, while maintaining the convenient spin column format for silica membrane-based DNA cleanup. This is a high-capacity version of our Monarch Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130). The Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) efficiently purifies and concentrates up to 100 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as RCA, RE digestion, labeling, or exonuclease treatment. The high capacity and rapid process time make the kit an excellent choice for cleanup of templates generated by cell-free DNA synthesis workflows. Modified protocols enabling cleanup of 100 µg amounts of oligonucleotides, genomic DNA, or RCA products make this kit a workhorse for multiplexed, high-throughput workflows requiring large amounts of starting materials. The kit features precision-engineered spin columns uniquely designed to enable low elution volumes (50 μl), resulting in highly concentrated DNA suitable for downstream applications such as in vitro transcription (IVT), genome editing, or viral vector transfection.
The kit includes spin columns, collection tubes, buffers, and other reagents, all compactly packaged. The spin columns are compatible with microcentrifuges, centrifuges, and vacuum manifolds, without additional plastic accessories. Manufactured using state-of-the-art automation, Monarch spin columns ensure high quality and consistency. The optimized buffer system helps ensure efficient DNA binding and purification, without the need to adjust pH.
Monarch kits are more eco-friendly than other options from leading suppliers, reducing plastic usage in the columns and bottles while maintaining structural integrity for centrifugation or vacuum manifolds.
Properties | |
---|---|
Purification format | Spin column |
DNA Sample | DNA from PCR and other enzymatic reactions (e.g., restriction digests, RCA, labeling, exonuclease treatment, ligations) |
ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol |
|
Genomic DNA and large size DNA (>25 kb) or RCA reaction cleanup using the supplemental protocols |
|
Typical Recovery | 70-90% |
DNA Purity | A260/280 > 1.8 and A260/230 > 1.8 |
Nucleic Acid Binding Capacity | Up to 100 μg of DNA |
Elution Volume | 50-200 μl |
DNA Size Range | Standard protocol: 40 bp – 25 kb |
Oligonucleotide Cleanup protocol: ssDNA >12 nt and dsDNA >10 nt |
|
Protocol Time | 9 minutes of spin and incubation time |
Compatible Downstream Applications | Ligation, in vitro transcription (IVT), genome editing, viral transfection, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |







- This product is related to the following categories:
- PCR & DNA Cleanup,
- Nucleic Acid Purification,
- This product can be used in the following applications:
- Nucleic Acid Purification
Kit Components
Kit Components
The following reagents are supplied with this product:
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Properties & Usage
Materials Required but not Supplied
- Isopropanol
- Ethanol
Features
- High Performance: Achieve high yields (up to 100 μg) and high purity in the purification, cleanup, and concentration of DNA, with the capability to remove short primers, detergents, and other low-molecular-weight reaction components (e.g. nucleotides, DMSO, betaine).
- High Concentration: Elute in very small volumes, in as little as 50 μl for elution, allowing for highly concentrated DNA.
- High Compatibility: Designed for maximum compatibility with microcentrifuges and vacuum manifolds using a spin column format. No extra accessories or plastics required.
- Broad Utility: Purify large (> 25 kb) or small DNA fragments (< 40 bp), including oligonucleotides, with this single kit, using the modified protocols provided.
- Unique Design: Spin column features a unique design that enables elution in low volumes and minimizes buffer retention and contaminant carryover.
- Optimized: Buffer system is optimized without the need to adjust pH.
- Application Compatibility: Purified DNA is ready for downstream molecular applications, such as in vitro transcription template, restriction digests, DNA sequencing, ligation, amplification, and other enzymatic reactions.
Related Products
Companion Products
Protocols, Manuals & Usage
Protocols
- Buffer Preparation Guidance for Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)
- Standard DNA Cleanup Protocol Using Centrifugation with the Monarch Spin High-Capacity DNA Cleanup Kit
- Standard DNA Cleanup Protocol Using Vacuum Manifolds with the Monarch Spin High-Capacity DNA Cleanup Kit (NEB #T1135)
- Oligonucleotide Cleanup Protocol using Centrifugation for the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)
- Protocol for genomic DNA (> 25 kb) cleanup using the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)
- Protocol for RCA reaction cleanup using the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)
Manuals
FAQs & Troubleshooting
FAQs
- Can I purchase additional Monarch® columns and collection tubes for the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135) separately?
- Can I use water to elute the DNA when using Monarch® Kits?
- What is the smallest volume of elution buffer that can be used with the Monarch® Spin Columns S3A (NEB #T1157)?
- What is the composition of each buffer provided with the Monarch® Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)?
- What is the maximum binding capacity of the Monarch® Spin Columns S3A provided with the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)?
- What factors affect the (A260/230) ratio when using the Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135)?
- Do you have any recommendations for the purification of single-stranded DNA (ssDNA) when using the Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135)?
- What size DNA can be purified with the Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135)?
- Are Monarch® Spin Columns S3A (NEB #T1157) compatible with vacuum manifolds?
- Can I use the Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135) to purify oligonucleotides and other DNA fragments?
- Can I use Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135) to purify circular DNA?
- Can I use Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135) to purify DNA > 25 kb or genomic DNA?
- Are the Monarch® Spin Columns S3A (NEB #T1157) compatible with the QIAcube® system?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Monarch® Buffer BZ
Monarch® Buffer WZ
Monarch® Buffer EY
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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The supporting documents available for this product can be downloaded below.