SRC-3 (5E11) Rabbit mAbProduct information
SRC-3 (5E11) Rabbit mAb
|100 µl (10 western blots)||-||Unavailable in your region|
Product Pathways - Nuclear Receptor Signaling
SRC-3 (5E11) Rabbit mAb #2126
|2126S||100 µl (10 western blots)||---||In Stock||---|
|2126||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||160||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq
Directions For Use
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Specificity / Sensitivity
SRC-3 (5E11) Rabbit mAb detects endogenous levels of total SRC-3 protein (all isoforms). The antibody does not cross-react with other SRC proteins, including SRC-1 and SRC-2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SRC-3 protein.
Western blot analysis of cell lysates from A549, HeLa and Jurkat cells using SRC-3 (5E11) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 minutes and SRC-3 (5E11) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across CCND1, a known target gene of SRC3 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 minutes and either SRC-3 (5E11) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CyclinD1 Promoter Primers #12531, human CTSD promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).
- Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
- Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
- Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
- Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
- Spencer, T.E. et al. (1997) Nature 389, 194-198.
- Chen, H. et al. (1997) Cell 90, 569-580.
- Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
- Chen, D. et al. (1999) Science 284, 2174-2177.
- Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
- Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
- Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
- Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
- Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
- Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.
- Luderer, H.F. et al. (2011) J Biol Chem 286, 18444-51. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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NEBNext is a registered trademark of New England Biolabs, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.