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Cell lysis, tissue disruption and homogenization are common Sonicator applications. The ultrasonic energy output of each Sonicator model is adjustable and sonication parameters can be optimized according to your process requirements. Amplitude (intensity) can be lowered in order to gently process fragile cells, prevent organelles from being damaged or decrease degradation of enzymes. Amplitude can also be increased to powerful levels to process difficult cell types (i.e. E.coli, yeast, algae, heart/skin tissue).
The choice of which Sonicator and probe size work best depends upon the sample volume range to be processed. Qsonica offers a variety of Sonicators and accessories which allows you to choose a system that fits both your application and budget.
Relationship between Sample Volume and Probe Size - Selecting the proper size probe is extremely important. Each probe has a recommended sample volume range. See the chart below for guidance. For example the ½” probe can process approximately 20-250ml...
|Tip Diameter||Processing Volume Range|
|1/16" (2mm)||200ul - 2ml|
|5/64" (2mm)||200ul - 2ml|
|1/8" (3mm)||1ml - 15ml|
|1/4" (6mm)||5ml - 50ml|
|1/2" (12mm)||20ml - 250ml|
|3/4" (19mm)||100ml - 500ml|
|1" (25mm)||200ml - 1,000ml|
|1” with booster||500ml - 1,500ml|
Visit the FAQ section for answers to more questions about sonication.