OneTaq® DNA Polymerases

 
An optimized blend of Taq and Deep VentR™ DNA polymerases, OneTaq® and OneTaq® Hot Start DNA Polymerases from New England Biolabs (NEB) offer robust amplification across a wide range of templates. The 3´–5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robustness of Taq, and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products.
Additionally, OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. Both polymerases are available in master mix and Quick-Load® master mix formats. 
 
OneTaq Quick-Load DNA Polymerase is an optimized, ready-to-use blend of Taq and Deep VentR DNA Polymerases ideally suited to “standard” PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies, cDNA products etc. It is supplied with a density and tracking dye containing 5x OneTaq Quick-Load Reaction Buffer for direct gel loading in addition to the regular “colorless” 5x OneTaq Reaction Buffer.

In contrast to chemically modified or antibody-based hot start polymerases, NEB's OneTaq Hot Start utilizes aptamer technology. This unique modified oligonucelotide binds to the polymerase through non-covalent interactions, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature.

OneTaq DNA Polymerase 

OneTaq Hot Start DNA Polymerase 

OneTaq 2X Master Mix with Standard Buffer 

OneTaq 2X Master Mix with GC Buffer 

OneTaq Hot Start 2X Master Mix with Standard Buffer 

OneTaq Hot Start 2X Master Mix with GC Buffer 

OneTaq Quick-Load 2X Master Mix with Standard Buffer 

OneTaq Quick-Load 2X Master Mix with GC Buffer 

OneTaq Hot Start Quick-Load 2X Master Mix, Standard Buffer 

OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer 

OneTaq Quick-Load DNA Polymerase 

 
Sample request OneTaq

 
See what our customers are saying about OneTaq® DNA Polymerases. Do you want to test yourself? Fill in your details below and receive a OneTaq DNA Polymerase sample**. 

**This free sample can be requested by laboratory from the Netherlands, Belgium and Luxembourg until June 30, 2017. No cash or cash equivalent. Offer may not be applied to existing, pending or prior orders. Free samples cannot be requests by current users

OneTaq® DNA Polymerases Feedback

The questionaire results thus far have been tallied and the numbers say it all:

• 89% reported Good or Excellent Yield 
• 88% reported Good or Excellent Purity 
• 90% reported Good or Excellent Overall Performance 
• 94% reported Good or Excellent Ease-of-Use

"We have switched over to using OneTaq for our routine PCR. It works well and is priced well." - RESEARCH SUPPORT SPECIALIST II, CORNELL UNOVERSITY

"Works like a charm with little to no standardization." - POST-DOCTORAL FELLOW, DANA FARBER CANCER INSTITUTE

"This is the most effective enzyme I have ever used." - GABRIELLA MIKLOSSY POSTDOCTORAL FELLOW, SUNY UPSATE MEDICAL UNIVERSITY

"It's easy to use, does the work and that's why we buy it all the time." - NIR HANIN, GRADUATE STUDENT, BAR-ILAN UNIVERSITY

"I liked it so much I bought a tube the next day." - TOM SMINK, RESEARCH TECHNICIAN, WEIS CENTER FOR RESEARCH

"I was able to get equally robust amplification from genomic DNA at all Tm's tested in gradient PCR with a new set of primers!" - CHIRANTHANI SUMANASEKERA, PHD, POSTDOCTORAL FELLOW, VANDERBILT UNIVERSITY SCHOOL OF MEDICINE

"This is the best Taq I have ever used. I love it." - XUDONG CHENG, POSTDOCTORAL FELLOW, FEINBERG SCHOOL OF MEDICINE, NORTHWESTERN UNIVERSITY

"Good yield, sample worked great!" - PREM SIDDHARTH GUNAMALAI, RESEARCH TECHNICIAN III, BAYLOR COLLEGE OF MEDICINE/UNITED STATES DEPARTMENT OF AGRICULTURE

"Easy to use, good performance!" - LINGYU WANG, GRADUATE STUDENT, UNIVERSITY OF MIAMI