Product Pathways - Cell Cycle / Checkpoint
PCNA (PC10) Mouse mAb (PE Conjugate) #8607
|8607S||100 µl (50 tests)||---||In Stock||---|
|8607||carrier free and custom formulation / quantity||email request|
|F||1:50||Human, Mouse, Rat, Monkey, Bovine, Pig||Endogenous||Mouse IgG2a|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: F=Flow Cytometry
Specificity / Sensitivity
PCNA (PC10) Mouse mAb (PE Conjugate) detects endogenous levels of total PCNA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PCNA (PC10) Mouse mAb #2586.
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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