Product Pathways - Apoptosis
Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617
|8617S||100 µl (50 tests)||---||In Stock||---|
|8617||carrier free and custom formulation / quantity||email request|
|IF-F||1:50||Human, Mouse, Rat, Monkey||Endogenous||Mouse IgG2a|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of lamin A and C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human lamin A protein.
Confocal immunofluorescent analysis of normal rat brain using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).
Flow cytometric analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Lamin A/C (4C11) Mouse mAb #4777.
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).
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- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.