Product Pathways - Cell Cycle / Checkpoint
Phospho-Rb (Ser608) (D10F2) Rabbit mAb #8147
|8147S||100 µl (10 western blots)||---||In Stock||---|
|8147||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Phospho-Rb (Ser608) (D10F2) Rabbit mAb recognizes endogenous levels of Rb protein only when phosphorylated at Ser608.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser608 of human Rb protein.
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-Rb (Ser608) (D10F2) Rabbit mAb (upper) or Rb (D20) Rabbit mAb #9313 (lower).
Western blot analysis of extracts from WI-38 cells, serum-starved for 3 days (-) or serum-starved for 3 days followed by treatment with 10% serum for 2 days (+), using Phospho-Rb (Ser608) (D10F2) Rabbit mAb.
Immunoprecipitation of phospho-Rb (Ser608) from WI-38 cell extracts using Phospho-Rb (Ser608) (D10F2) Rabbit mAb (lane 2). Western blot was performed using the same antibody. Lane 1 is 10% input.
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
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- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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