Product Class: Kit

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

The S size of this product has been discontinued as of September 2018. The L size will still be available. 

Product Introduction

The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent® includes enzymatic DNA fragmentation in a streamlined, high-performance library prep workflow.

  • High yield 
  • Fast, simple workflow – under 2 hours, with minimal hands-on time 
  • Compatible with broad range of sample input (10 ng –1 μg)
Catalog # Size Concentration
E6285L 50.0 reactions

Protocols, Manuals & Usage

Protocols

  1. NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. What sequencing platform can I use the NEBNext Fast DNA  Library Prep Sets for Ion Torrent reagents for?
  2. What type of starting material can be used with NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent?
  3. How much starting material do I need to use when preparing libraries using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent?
  4. Does the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent work well for GC-rich DNA and AT-rich DNA?
  5. Can I use the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent for preparing RNA?
  6. What methods for size selection can be used with Ion Torrent Library Preparation Kits?
  7. Are these reagents available in a customized format, larger format or 96 well formats?
  8. What do I do if my fragmented DNA is too short?
  9. The peak on my electropherogram trace is broader than I would like. How can I make the size range more narrow?
  10. Do I really need to vortex the fragmentation reactions, as described in the protocol? Will pipetting up and down work just as well?
  11. What methods for size selection can be used with Ion Torrent Library Preparation Kits?
  12. What do I do if my DNA is not being fragmented?
  13. Are DNA adaptors or oligonucleotides included in the NEBNext® products?
  14. Are NEBNext® products validated in Next Generation Sequencing workflows?

Other Products You May Be Interested In