Product Pathways - Cytoskeletal Signaling
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 555 Conjugate) #3478
|3478S||100 µl (50 tests)||---||In Stock||---|
|3478||carrier free and custom formulation / quantity||email request|
|IF-IC||1:50||Human, Rat, Monkey||Endogenous||Mouse IgG1|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells. The antibody was conjugated to Alexa Fluor®
555 under optimal conditions with an F/P ratio of 2-6.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluroescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Pan-Keratin (C11) Mouse mAb #4545.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.