Product Class: Other

USER® Enzyme
neb31 cloned at NEB recombinant 37 No

USER Enzyme has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10233215.

Thermolabile USER II Enzyme (NEB #M5508) and Thermostable USER III Enzyme (NEB #M5509) are available.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Product Introduction

USER (Uracil-Specific Excision Reagent) Enzyme (1) generates a single nucleotide gap at the location of a uracil residue.

Applications of USER include:

  • Directional RNA Seq
  • NEBNext adaptor cleavage
  • USER cloning

 

Figure 1: USER Enzyme

Uracil DNA glycosylase (UDG) catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3´ and 5´ sides of the abasic site so that base-free deoxyribose is released. USER (Uracil-Specific Excision Reagent) Enzyme, combines these two enzymatic activities to generate a single nucleotide gap at the location of a uracil residue.


Catalog # Size Concentration
M5505S 50.0 units 1000 units/ml
M5505L 250.0 units 1000 units/ml

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Will the USER Enzyme work in rCutSmart buffer?
  2. Where can I find information or a protocol for cloning with USER® Enzyme?
  3. During an NEBNext DNA library prep, which enzyme cleaves NEBNext DNA loop adaptors and uracil-containing single stranded loops?
  4. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

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