Product Class: Control DNA

Lambda DNA (N6-methyladenine-free)

Catalog #SizeConcentration
N3013S250 μg500 μg/ml
N3013L1,250 μg500 μg/ml

Description

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (N6-methyladenine-free) is 48,502 base pairs in length.

Source

The phage is isolated from a heat-inducible E. coli (dam-) lambda lysogen. The DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

Properties and Usage

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Supporting Documents

Material Safety Datasheets

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Notes

  1. The advantage of this DNA is that it gives complete digests with those restriction endonucleases (BclI, MboI, DpnII, etc.) whose recognition sites are partially modified by most E. coli strains.

References

  1. Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
  1. Is the Lambda DNA (N3011) methylated?