Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (N6-methyladenine-free) is 48,502 base pairs in length.
The phage is isolated from a heat-inducible E. coli
(dam-) lambda lysogen. The DNA is isolated from
purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0)
and 1 mM EDTA.
Properties and Usage
10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at firstname.lastname@example.org or fill out the Technical Support Form for appropriate document.